Super‐Resolution Ultrasound Imaging of Skeletal Muscle Microvascular Dysfunction in an Animal Model of Type 2 Diabetes
| العنوان: | Super‐Resolution Ultrasound Imaging of Skeletal Muscle Microvascular Dysfunction in an Animal Model of Type 2 Diabetes |
|---|---|
| المؤلفون: | Philip W. Shaul, Jun Peng, Kenneth Hoyt, Debabrata Ghosh, Katherine G. Brown, Chieko Mineo, Shashank R. Sirsi |
| المصدر: | Journal of Ultrasound in Medicine. 38:2589-2599 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, Contrast Media, Type 2 diabetes, Hindlimb, Article, 030218 nuclear medicine & medical imaging, Mice, 03 medical and health sciences, Animal data, 0302 clinical medicine, medicine, Animals, Radiology, Nuclear Medicine and imaging, Muscle, Skeletal, Ultrasonography, 030219 obstetrics & reproductive medicine, Radiological and Ultrasound Technology, business.industry, Ultrasound, Skeletal muscle, Blood flow, Image Enhancement, medicine.disease, Mice, Inbred C57BL, Disease Models, Animal, medicine.anatomical_structure, Diabetes Mellitus, Type 2, Microvessels, Microbubbles, Nuclear medicine, business, Perfusion |
| الوصف: | OBJECTIVES—: To evaluate the use of super-resolution ultrasound (SR-US) imaging for quantifying microvascular changes in skeletal muscle using a mouse model of type 2 diabetes. METHODS—: Study groups were young, standard chow–fed male C57BL/6J mice (lean group) and high fat diet–fed older mice (obese group). After an overnight fast, dynamic contrast-enhanced US imaging was performed on the proximal hind limb adductor muscle group for 10 minutes at baseline and again at 1 and 2 hours during administration of a hyperinsulinemic-euglycemic clamp. Dynamic contrast-enhanced US images were collected on a clinical US scanner (Acuson Sequoia 512; Siemens Healthcare, Mountain View, CA) equipped with a 15L8 linear array transducer. Dynamic contrast-enhanced US images were processed with a spatiotemporal filter to remove tissue clutter. Individual microbubbles were localized and counted to create an SR-US image. A frame-by-frame analysis of the microbubble count was generated (ie, time-microbubble count curve [TMC]) to estimate tissue perfusion and microvascular blood flow. The conventional time-intensity curve (TIC) was also generated for comparison. RESULTS—: In vivo SR-US imaging could delineate microvascular structures in the mouse hind limb. Compared with lean animals, insulin-induced microvascular recruitment was attenuated in the obese group. The SR-US-based TMC analysis revealed differences between lean and obese animal data for select microvascular parameters (P < .04), which was not true for TIC-based measurements. Whereas the TMC and TIC microvascular parameters yielded similar temporal trends, there was less variance associated with the TMC-derived values. CONCLUSIONS—: Super-resolution US imaging is a new modality for measuring the microvascular properties of skeletal muscle and dysfunction from type 2 diabetes. |
| تدمد: | 1550-9613 0278-4297 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ebc0cb863507a61cf36a2e1d5ce032dTest https://doi.org/10.1002/jum.14956Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9ebc0cb863507a61cf36a2e1d5ce032d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15509613 02784297 |
|---|