No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats
| العنوان: | No Mutations of Lysophosphatidic Acid Receptor Genes in Lung Adenocarcinomas Induced by N-Nitrosobis(2-hydroxypropyl)amine in Rats |
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| المؤلفون: | Ayumi Koshino, Megumu Tsujino, Naoko Wakabayashi, Hiroko Ikeda, Toshifumi Tsujiuchi, Nobuyuki Fukushima, Masaki Tajiri, Midori Taki |
| المصدر: | Journal of Toxicologic Pathology |
| بيانات النشر: | Japanese Society of Toxicologic Pathology, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, lysophosphatidic acid receptor, Short Communication, Single-strand conformation polymorphism, Gene mutation, Biology, lung adenocarcinoma, Toxicology, medicine.disease_cause, Molecular biology, Pathology and Forensic Medicine, chemistry.chemical_compound, chemistry, Cell surface receptor, Apoptosis, Lysophosphatidic acid, medicine, N-nitrosobis(2-hydroxypropyl)amine, rat, mutation, Receptor, Carcinogenesis, Gene |
| الوصف: | Lysophosphatidic acid (LPA) is a bioactive phospholipid that stimulates cell proliferation and migration, and protects cells from apoptosis. It interacts with specific G protein-coupled transmembrane receptors. Recently, frequent mutations of the LPA receptor-1 (LPA1) gene were detected in rat lung adenocarcinomas induced by N-nitrosobis(2-hydroxypropyl)amine (BHP). In this study, to evaluate the involvement of other LPA receptor gene alterations during lung carcinogenesis, we investigated mutations of the LPA2, LPA3, LPA4 and LPA5 genes in lung adenocarcinomas induced by BHP in rats. Fifteen male Wistar rats, 6 weeks of age, were given 2000 ppm BHP in their drinking water for 12 weeks and then maintained without further treatment until sacrifice at 25 weeks, and 15 adenocarcinomas were obtained. Genomic DNAs were extracted from frozen tissues, and the LPA2, LPA3, LPA4 and LPA5 genes were examined for mutations, using polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) analysis. No mutations of LPA2, LPA3, LPA4 and LPA5 were detected in the 15 adenocarcinomas. These results suggest that alterations due to LPA2, LPA3, LPA4 and LPA5 gene mutations might not be involved in the development of lung adenocarcinomas induced by BHP in rats. |
| تدمد: | 1881-915X 0914-9198 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6932974d146a11c1276f6bf94f50ac41Test https://doi.org/10.1293/tox.23.63Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6932974d146a11c1276f6bf94f50ac41 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1881915X 09149198 |
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