Gallium enhances reconstructive properties of a calcium phosphate bone biomaterial
| العنوان: | Gallium enhances reconstructive properties of a calcium phosphate bone biomaterial |
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| المؤلفون: | Jean-Michel Bouler, Ivana Strazic Geljic, Pascal Janvier, Jean-Claude Scimeca, Nicolas Melis, Florian Boukhechba, Charlotte Mellier, Sébastien Schaub, Elise Verron, Jean-Pierre Laugier |
| المساهمون: | Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), GRAFTYS SA [Aix en Provence, France], Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Centre Commun de Microscopie Appliquée [Nice] (CCMA), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Nice University, CNRS, Graftys SA (Aix‐en‐Provence, France) and INSERM supported this work.This work was sponsored by Graftys SA, Convention CIFRE N°2011/1356., Jehan, Frederic, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Graftys SARL, Institute of Developmental Biology and Cancer (IBDC), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Centre Commun de Microscopie Appliquée (CCMA), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA) |
| المصدر: | Journal of Tissue Engineering and Regenerative Medicine Journal of Tissue Engineering and Regenerative Medicine, John Wiley & Sons Ltd., 2017, pp.854-866. ⟨10.1002/term.2396⟩ Journal of Tissue Engineering and Regenerative Medicine, 2017, 12, pp.854-866. ⟨10.1002/term.2396⟩ Journal of Tissue Engineering and Regenerative Medicine, John Wiley & Sons Ltd., 2018, 12 (2), pp.e854-e866. ⟨10.1002/term.2396⟩ |
| بيانات النشر: | Hindawi Limited, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Calcium Phosphates, 0301 basic medicine, Medicine (miscellaneous), bone reconstruction, bone repair quantification, calcium phosphate cements, gallium, gallium bone delivery, osteoclast/osteoblast crosstalk, second harmonic generation quantitative analysis, Biocompatible Materials, Gallium, Bone tissue, Bone remodeling, 0302 clinical medicine, Apatites, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Bone cell, Femur, Cells, Cultured, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Chemistry, [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology, Bone Cements, Biomaterial, Cell Differentiation, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], 3. Good health, medicine.anatomical_structure, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, 030220 oncology & carcinogenesis, Biomedical Engineering, [SDV.CAN]Life Sciences [q-bio]/Cancer, Bone healing, Bone resorption, Osseointegration, Biomaterials, 03 medical and health sciences, Osteoclast, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], medicine, Animals, Humans, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, Cell Shape, Cell Proliferation, Osteoblasts, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Rats, 030104 developmental biology, Bone Substitutes, Biomedical engineering |
| الوصف: | International audience; Calcium phosphate (CaP)-based biomaterials are commonly used in bone reconstructive surgery to replace the damaged tissue, and can also serve as vectors for local drug delivery. Due to its inhibitory action on osteoclasts, the semi-metallic element gallium (Ga) is used for the systemic treatment of disorders associated with accelerated bone resorption. As it was demonstrated that Ga could be incorporated in the structure of CaP biomaterials, we investigated the biological properties of Ga-loaded CaP biomaterials. Culturing bone cells on Ga-CaP, we observed a decrease in osteoclast number and a downregulation of late osteoclastic markers expression, while Ga-CaP upregulated the expression of osteoblastic marker genes involved in the maturation of bone matrix. We next investigated in vivo bone reconstructive properties of different Ga-loaded biomaterials using a murine bone defect healing model. All implanted biomaterials showed a good osseointegration into the surrounding host tissue, accompanied by a successful bone ingrowth and bone marrow reconstruction, as evidenced by histological analysis. Moreover, quantitative micro-computed tomography analysis of implants revealed that Ga enhanced total defect filling. Lastly, we took advantage for the first time of a particular mode of non-linear microscopy (second harmonic generation) to quantify in vivo bone tissue reconstruction within a CaP bone substitute. By doing so, we showed that Ga exerted a positive impact on mature organized collagen synthesis. As a whole, our data support the hypothesis that Ga represents an attractive additive to CaP biomaterials for bone reconstructive surgery. Copyright © 2017 John Wiley & Sons, Ltd. |
| وصف الملف: | application/pdf |
| تدمد: | 1932-7005 1932-6254 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73e570f531e55d1bb80e603ac459b2ceTest https://doi.org/10.1002/term.2396Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....73e570f531e55d1bb80e603ac459b2ce |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19327005 19326254 |
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