Interphotoreceptor matrix-poly(ϵ-caprolactone) composite scaffolds for human photoreceptor differentiation
| العنوان: | Interphotoreceptor matrix-poly(ϵ-caprolactone) composite scaffolds for human photoreceptor differentiation |
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| المؤلفون: | Petr Baranov, Andrew Michaelson, Joydip Kundu, Rebecca L Carrier, Michael Young |
| المصدر: | Journal of Tissue Engineering Journal of Tissue Engineering, Vol 5 (2014) |
| بيانات النشر: | SAGE Publications, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | medicine.medical_specialty, Biomedical Engineering, Medicine (miscellaneous), Interphotoreceptor matrix, Biology, Retina, interphotoreceptor matrix, lcsh:Biochemistry, Biomaterials, Extracellular matrix, chemistry.chemical_compound, Tissue engineering, polycaprolactone, Ophthalmology, medicine, lcsh:QD415-436, Retinal regeneration, photoreceptors, Retinal, 3. Good health, Transplantation, Fibronectin, medicine.anatomical_structure, chemistry, Biophysics, biology.protein, Original Article, sense organs |
| الوصف: | Tissue engineering has been widely applied in different areas of regenerative medicine, including retinal regeneration. Typically, artificial biopolymers require additional surface modification (e.g. with arginine–glycine–aspartate-containing peptides or adsorption of protein, such as fibronectin), before cell seeding. Here, we describe an alternative approach for scaffold design: the manufacture of hybrid interphotoreceptor matrix-poly (ϵ-caprolactone) scaffolds, in which the insoluble extracellular matrix of the retina is incorporated into a biodegradable polymer well suited for transplantation. The incorporation of interphotoreceptor matrix did not change the topography of polycaprolactone film, although it led to a slight increase in hydrophilic properties (water contact angle measurements). This hybrid scaffold provided sufficient stimuli for human retinal progenitor cell adhesion and inhibited proliferation, leading to differentiation toward photoreceptor cells (expression of Crx, Nrl, rhodopsin, ROM1). This scaffold may be used for transplantation of retinal progenitor cells and their progeny to treat retinal degenerative disorders. |
| تدمد: | 2041-7314 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f23556d5f2e5f74d0b07812909269daTest https://doi.org/10.1177/2041731414554139Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3f23556d5f2e5f74d0b07812909269da |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20417314 |
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