A procedure for the microinjection of plant cells and protoplasts
| العنوان: | A procedure for the microinjection of plant cells and protoplasts |
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| المؤلفون: | Roger Kemble, Daina H. Simmonds, Sharon Bird, Bin Huang, Wilfred A. Keller, Brian Miki |
| المصدر: | Journal of Tissue Culture Methods. 12:139-144 |
| بيانات النشر: | Springer Science and Business Media LLC, 1989. |
| سنة النشر: | 1989 |
| مصطلحات موضوعية: | General method, fungi, food and beverages, Cell Biology, Protoplast, Biology, Plant cell, Suspension culture, Pathology and Forensic Medicine, Cell biology, chemistry.chemical_compound, Microspore, chemistry, Cell culture, Botany, Agarose, Anatomy, Microinjection |
| الوصف: | This paper describes a general method suitable for the microinjection ofBrassica napus protoplasts, unicellular microspores, and multicellular microspores. By incorporating components taken from other methods, manual operations frequently involved in the microinjection of plant cells have been simplified and microinjection rates increased. The embedding of cells in agarose provides a simple alternative to the variety of sophisticated immobilization strategies devised for different plant cell types thereby reducing the manipulations often involved in the culture of microinjected cells. Use of an automatic microinjector eliminated the operation of fine control systems on manual injectors; however, precision in sample delivery was reduced. Analyses indicate that transformed tissues can be recovered from microinjected protoplasts and microspores at high frequencies. |
| تدمد: | 1573-0603 0271-8057 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::92754bfa6dc1576f229f9dd3aa9acb19Test https://doi.org/10.1007/bf01404440Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........92754bfa6dc1576f229f9dd3aa9acb19 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15730603 02718057 |
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