دورية
Infection with a periodontal pathogen induces procoagulant effects in human aortic endothelial cells
| العنوان: | Infection with a periodontal pathogen induces procoagulant effects in human aortic endothelial cells |
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| المؤلفون: | ROTH, G.A., MOSER, B., HUANG, S.J., BRANDT, J.S., HUANG, Y., PAPAPANOU, P.N., SCHMIDT, A.M., LALLA, E. |
| المصدر: | Journal of Thrombosis and Haemostasis; October 2006, Vol. 4 Issue: 10 p2256-2261, 6p |
| مستخلص: | Background:Multiple studies have demonstrated a link between periodontal infections and vascular disease. Porphyromonas gingivalis, a major periodontal pathogen, has been shown to adhere to and invade endothelial cells. Objective:In order to dissect mechanisms underlying these observations, we assessed the role of P. gingivalisinfection in modulating properties of endothelial cells linked to atherothrombosis. Methods:Primary human aortic endothelial cells (HAEC) were infected with either P. gingivalis381 or its non‐invasive fimbriae‐deficient mutant, DPG3. Markers of coagulation and thrombosis were assessed 8 h and 18 h postinfection in cell lysates and supernatants. Results:Infection with P. gingivalis381 significantly enhanced tissue factor expression and activity, and suppressed levels of tissue factor pathway inhibitor. Furthermore, P. gingivalisinfection decreased levels and activity of tissue plasminogen activator, and enhanced plasminogen activator inhibitor‐1 antigen and activity. Consistent with an important role for bacterial adhesion/invasion in this setting, infection with DPG3 failed to induce procoagulant properties in HAEC. Most of the above effects of P. gingivalis381 were more apparent at the later time point (18 h postinfection). This suggests that P. gingivalisinfection, rather than having an immediate and direct effect, might activate pathways that, in turn, trigger endothelial procoagulant mechanisms. Conclusions:Taken together these data demonstrate for the first time that infection with a periodontal pathogen induces procoagulant responses in HAEC. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 15387933 15387836 |
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| DOI: | 10.1111/j.1538-7836.2006.02128.x |