Combined exosomal RNA and circulating tumor DNA for epidermal growth factor mutation detection in non-small cell lung cancer
| العنوان: | Combined exosomal RNA and circulating tumor DNA for epidermal growth factor mutation detection in non-small cell lung cancer |
|---|---|
| المؤلفون: | Elisa Giovannetti, Vanesa Gregorc, Chiara Lazzari, Maria Giulia Cangi, Mariacarmela Santarpia, Alessandra Bulotta |
| المصدر: | Lazzari, C, Gregorc, V, Cangi, M G, Giovannetti, E, Bulotta, A & Santarpia, M 2018, ' Combined exosomal RNA and circulating tumor DNA for epidermal growth factor mutation detection in non-small cell lung cancer ', Journal of Thoracic Disease, vol. 10, pp. S4023-S4027 . https://doi.org/10.21037/jtd.2018.10.17Test Journal of thoracic disease (Online) 10 (2018): S4023–S4027. doi:10.21037/jtd.2018.10.17 info:cnr-pdr/source/autori:Lazzari C.; Gregorc V.; Cangi M.G.; Giovannetti E.; Bulotta A.; Santarpia M./titolo:Combined exosomal RNA and circulating tumor DNA for epidermal growth factor mutation detection in non-small cell lung cancer/doi:10.21037%2Fjtd.2018.10.17/rivista:Journal of thoracic disease (Online)/anno:2018/pagina_da:S4023/pagina_a:S4027/intervallo_pagine:S4023–S4027/volume:10 |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Adult, Male, Lung Neoplasms, DNA Mutational Analysis, Antineoplastic Agents, Exosomes, Sensitivity and Specificity, Circulating Tumor DNA, Epidermal growth factor, Carcinoma, Non-Small-Cell Lung, medicine, ROS1, Biomarkers, Tumor, Anaplastic lymphoma kinase, Humans, Mutation detection, Lung cancer, Aged, Acrylamides, business.industry, Liquid Biopsy, RNA, Genes, erbB-1, Middle Aged, medicine.disease, ErbB Receptors, Editorial, Pyrimidines, Circulating tumor DNA, Cancer research, Female, Non small cell, business |
| الوصف: | A major limitation of circulating tumor DNA (ctDNA) for somatic mutation detection has been the low level of ctDNA found in a subset of cancer patients. We investigated whether using a combined isolation of exosomal RNA (exoRNA) and cell-free DNA (cfDNA) could improve blood-based liquid biopsy for EGFR mutation detection in non-small-cell lung cancer (NSCLC) patients.Matched pretreatment tumor and plasma were collected from 84 patients enrolled in TIGER-X (NCT01526928), a phase 1/2 study of rociletinib in mutant EGFR NSCLC patients. The combined isolated exoRNA and cfDNA (exoNA) was analyzed blinded for mutations using a targeted next-generation sequencing panel (EXO1000) and compared with existing data from the same samples using analysis of ctDNA by BEAMing.For exoNA, the sensitivity was 98% for detection of activating EGFR mutations and 90% for EGFR T790M. The corresponding sensitivities for ctDNA by BEAMing were 82% for activating mutations and 84% for T790M. In a subgroup of patients with intrathoracic metastatic disease (M0/M1a; n = 21), the sensitivity increased from 26% to 74% for activating mutations (P = 0.003) and from 19% to 31% for T790M (P = 0.5) when using exoNA for detection.Combining exoRNA and ctDNA increased the sensitivity for EGFR mutation detection in plasma, with the largest improvement seen in the subgroup of M0/M1a disease patients known to have low levels of ctDNA and poses challenges for mutation detection on ctDNA alone.NCT01526928. |
| اللغة: | English |
| تدمد: | 2072-1439 0152-6928 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a85b54e65ba6cced812d1e88976ca0d2Test https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85057399178&origin=inwardTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a85b54e65ba6cced812d1e88976ca0d2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20721439 01526928 |
|---|