Optimal viral strategies for bypassing RNA silencing
| العنوان: | Optimal viral strategies for bypassing RNA silencing |
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| المؤلفون: | Guillermo Rodrigo, Santiago F. Elena, Javier Carrera, Alfonso Jaramillo |
| المساهمون: | Instituto de Biología Molecular y Celular de Plantas, Universitat Politècnica de València (UPV), ITACA, Laboratoire de Biochimie de l'Ecole polytechnique (BIOC), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), Programme d'Épigénomique, Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Santa Fe Institute, Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X) |
| المصدر: | Interface Interface, 2011, 8 (55), pp.257-68. ⟨10.1098/rsif.2010.0264⟩ Interface, Mitchell Publ., 2011, 8 (55), pp.257-68. ⟨10.1098/rsif.2010.0264⟩ RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname Digital.CSIC. Repositorio Institucional del CSIC Journal of The Royal Society Interface |
| بيانات النشر: | The Royal Society, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | 0106 biological sciences, Small interfering RNA, RNA induced silencing complex, Virus replication, Virus-host interaction, 01 natural sciences, Biochemistry, Mathematical model, Suppression, Genetic, RNA interference, MESH: RNA, Small Interfering, Immune systems, MESH: Models, Genetic, RNA, Small Interfering, Transcription-translation tradeoff, Double-stranded RNA, MESH: Suppression, Genetic, Research Articles, RNA transcription, MESH: Evolution, Molecular, Model-driven, Genetics, 0303 health sciences, Protein components, Gene silencing, Eukaryota, Argonaute, Virus, 3. Good health, Cell biology, RNA silencing, Viruses, RNA Interference, Viral RNA, Transcription, MESH: RNA Viruses, Biotechnology, Optimization, Systems and synthetic biology, RNA-induced silencing complex, MESH: RNA Interference, Trans-acting siRNA, Post-transcriptional, Biomedical Engineering, Biophysics, Defence mechanisms, Bioengineering, Mechanism of action, Biology, Article, Evolution, Molecular, Biomaterials, Viral Proteins, 03 medical and health sciences, Suppressor gene, Design Principles, RNA Viruses, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Designing principle, Synthetic biology, 030304 developmental biology, RNA silencing pathway, Sequence-specific manner, RNA translation, Models, Genetic, Virus cell interaction, Proteins, RNA, Working principles, Nonhuman, Virus evolution, MESH: Viral Proteins, Silencing suppression, 010606 plant biology & botany |
| الوصف: | The RNA silencing pathway constitutes a defense mechanism highly conserved in eukaryotes, especially in plants, where the underlying working principle relies on the repressive action triggered by the intracellular presence of double-stranded RNAs. This immune system performs a post-transcriptional suppression of aberrant mRNAs or viral RNAs by small interfering RNAs that are directed towards their target in a sequencespecific manner. However, viruses have evolved strategies to escape from silencing surveillance while promoting their own replication. Several viruses encode suppressor proteins that interact with different elements of the RNA silencing pathway and block it. The different suppressors are not phylogenetically nor structurally related and also differ in their mechanism of action. Here, we adopt a model-driven forward-engineering approach to understand the evolution of suppressor proteins and, in particular, why viral suppressors preferentially target some components of the silencing pathway. We analyzed three strategies characterized by different design principles: replication in the absence of a suppressor, suppressors targeting the first protein component of the pathway and suppressors targeting the small interfering RNAs. Our results seed light into the question of whether a virus must opt for devoting more time into transcription or into translation and on which would be the optimal step of the silencing pathway to be targeted by suppressors. In addition, we discussed the evolutionary implications of such designing principles. This work was supported by the Spanish Ministerio de Ciencia e Innovación grants BFU2009- 06993 to S.F.E. and TIN-2006-12860 to A.J. And by FP6-NEST-043340 (BioModularH2), FP7- ICT-043338 (Bactocom), FP7-KBBE-212894 (Tarpol), the Structural Funds of the European Regional Development Fund, the ATIGE-Genopole and the Foundation pour la Recherche Medicale grants (all to A.J.). J.C, G.R. and A.J. also acknowledge the HPC-Europa program (RII3- CT-2003-506079). G.R. was supported by a graduate fellowship from the Generalitat Valenciana and an EMBO Short-term fellowship. |
| وصف الملف: | application/pdf |
| تدمد: | 1742-5662 1742-5689 0163-6626 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a20ef84f376e6224b61e2f1303738a69Test https://doi.org/10.1098/rsif.2010.0264Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a20ef84f376e6224b61e2f1303738a69 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17425662 17425689 01636626 |
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