التفاصيل البيبلوغرافية
| العنوان: |
On-line Solid-Phase Extraction-Liquid Chromatographic Method for the Determination of Carbofuran and 7-Phenolcarbofuran in Cervical-Uterine Epithelial Tissue |
| المؤلفون: |
Vera-Avila,Luz E., Macías-Mendoza,Diana, Covarrubias-Herrera,Rosario, Velázquez-Ramírez,José G. |
| المصدر: |
Journal of the Mexican Chemical Society v.49 n.1 2005 |
| بيانات النشر: |
Sociedad Química de México A.C. |
| سنة النشر: |
2005 |
| مصطلحات موضوعية: |
Carbofuran, 7-phenolcarbofuran, trace analysis, cervical-uterine tissue |
| الوصف: |
A method for the trace level determination of carbofuran and 7-phenolcarbofuran in cervical-uterine epithelial tissue samples is proposed. The finely chopped tissue is methanol extracted in an ultrasonic bath; the extract is evaporated, diluted with water and acidified to eliminate hydrophobic impurities by precipitation. Preconcentration and additional cleanup are carried out in a polymeric (PLRP-S) precolumn by loading the filtrated extract and successively washing with acetonitrile-water 5:95 (v/v) and 0.01 M aqueous ammonia. The precolumn is finally on-line eluted and analysed by reverse-phase HPLC with UV detection at 280 nm. Recoveries >90% and relative standard deviation of ∼12% were obtained in the analysis of non-contaminated tissue samples spiked at 0.2 µg/g of the pesticide and metabolite. Absolute limits of detection were 20 ng of analyte in a cervical biopsy (150-950 mg of epithelial tissue). Application to the analysis of three samples from countrywomen exposed to carbofuran revealed the probable presence of 7-phenolcar-bofuran in two of them. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
text/html |
| اللغة: |
English |
| الإتاحة: |
http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-249X2005000100006Test |
| حقوق: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.A4647ECF |
| قاعدة البيانات: |
BASE |
