The distribution of 5α-dihydrotestosterone (DHT), and 3α-androstanediol (3α-diol), 17β-hydroxyandrogens, and 5α-androstanedione, and androsterone, 17-ketoandrogens was determined by measuring their content by radioimmunoassay in subcellular components of prostate from normal adult dogs. DHT followed by 3α-diol was the most abundant 5α-reduced androgen measured in dog prostate. DHT appears to concentrate particularly in the nucleus whereas the other androgens measured do not show such a selective localization.