التفاصيل البيبلوغرافية
| العنوان: |
Proteome and metabolome profiling of wild-type and YCA1-knock-out yeast cells during acetic acid-induced programmed cell death. |
| المؤلفون: |
Longo, Valentina1, Ždralević, Maša2, Guaragnella, Nicoletta2, Giannattasio, Sergio2, Zolla, Lello1 zolla@unitus.it, Timperio, Anna Maria1 timperio@unitus.it |
| المصدر: |
Journal of Proteomics. Oct2015, Vol. 128, p173-188. 16p. |
| مصطلحات موضوعية: |
*YEAST, *PROTEOMICS, *CELL death, *ACETIC acid, *APOPTOSIS |
| مستخلص: |
Caspase proteases are responsible for the regulated disassembly of the cell into apoptotic bodies during mammalian apoptosis. Structural homologues of the caspase family (called metacaspases) are involved in programmed cell death in single-cell eukaryotes, yet the molecular mechanisms that contribute to death are currently undefined. Recent evidence revealed that a programmed cell death process is induced by acetic acid (AA-PCD) in Saccharomyces cerevisiae both in the presence and absence of metacaspase encoding gene YCA1 . Here, we report an unexpected role for the yeast metacaspase in protein quality and metabolite control. By using an “omics” approach, we focused our attention on proteins and metabolites differentially modulated en route to AA-PCD either in wild type or YCA1 -lacking cells. Quantitative proteomic and metabolomic analyses of wild type and Δyca1 cells identified significant alterations in carbohydrate catabolism, lipid metabolism, proteolysis and stress-response, highlighting the main roles of metacaspase in AA-PCD. Finally, deletion of YCA1 led to AA-PCD pathway through the activation of ceramides, whereas in the presence of the gene yeast cells underwent an AA-PCD pathway characterized by the shift of the main glycolytic pathway to the pentose phosphate pathway and a proteolytic mechanism to cope with oxidative stress. Significance The yeast metacaspase regulates both proteolytic activities through the ubiquitin–proteasome system and ceramide metabolism as revealed by proteome and metabolome profiling of YCA1 -knock-out cells during acetic-acid induced programmed cell death. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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