Starvation Induces Phase-Specific Changes in the Proteome of Mouse Small Intestine
| العنوان: | Starvation Induces Phase-Specific Changes in the Proteome of Mouse Small Intestine |
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| المؤلفون: | Johan Renes, Kaatje Lenaerts, Freek G. Bouwman, Edwin C. M. Mariman, Wouter H. Lamers, Milka Sokolović |
| المساهمون: | Other departments, Amsterdam Gastroenterology Endocrinology Metabolism, Medical Biology, Humane Biologie, Anatomie en Embryologie, RS: NUTRIM School of Nutrition and Translational Research in Metabolism |
| المصدر: | Journal of proteome research, 5(9), 2113-2122. American Chemical Society Journal of Proteome Research, 5(9), 2113-2122. American Chemical Society |
| بيانات النشر: | American Chemical Society (ACS), 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Starvation, GPX3, Mucin, General Chemistry, Biology, Proteomics, Biochemistry, Small intestine, Cell biology, medicine.anatomical_structure, Proteome, medicine, medicine.symptom, Starvation response, Carbonic anhydrase 3 |
| الوصف: | Food deprivation results in metabolic, structural, and functional changes in the small intestine that influences gut mucosal integrity, epithelial cell proliferation, mucin synthesis, and other processes. The underlying mechanisms are still unclear, which lead to the study of molecular effects of short-term and long-term starvation in the intestine of mice. A comparative proteomics approach, combining two-dimensional gel electrophoresis with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, was used to identify intestinal proteins whose expression is changed under different starvation conditions (0, 12, 24, and 72 h). In total, the expression levels of 80 protein spots changed significantly between the different groups. The results demonstrate that after 12 h of starvation, mainly proteins involved in glycolysis and energy metabolism show decreased expression levels. Starvation for 24 h results in a down-regulation of proteins involved in protein synthesis and amino acid metabolism. Simultaneously, proteins with a protective role, e.g., reg I and II, glutathione peroxidase 3, and carbonic anhydrase 3, are clearly up-regulated. The last starvation phase (72 h) is characterized by increased ezrin expression, which may enhance villus morphogenesis critical for survival. Together, these results provide novel insights in the intestinal starvation response and may contribute to improved nutritional support during conditions characterized by malnutrition. |
| تدمد: | 1535-3907 1535-3893 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cc22139f419108a8eeced5e853efd75Test https://doi.org/10.1021/pr060183Test+ |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1cc22139f419108a8eeced5e853efd75 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15353907 15353893 |
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