التفاصيل البيبلوغرافية
| العنوان: |
PTEN 介导紫草素抑制人增生性瘢痕成纤维细胞的 胶原沉积. (Chinese) |
| العنوان البديل: |
Shikonin inhibits collagen deposition of human hypertrophic scar fibroblasts via PTEN. (English) |
| المؤلفون: |
唐玉婷, 马芳, 贺茜, 王建军, 杨安宁, 吴凯, 焦运, 白志刚, 姜怡邓, 沈江涌 |
| المصدر: |
Journal of Practical Medicine / Shiyong Yixue Zazhi; 6/10/2023, Vol. 39 Issue 11, p1382-1388, 7p |
| الملخص (بالإنجليزية): |
Objective To investigate the effect of shikonin on the collagen deposition of human hypertrophic scar fibroblasts from the perspective of PTEN. Methods Hematoxylin-eosin staining and Masson staining were used to identify hypertrophic scar tissue. Immunofluorescence was used to identify fibroblasts. The relative expression level of PTEN was detected by Western blot at the tissue level. Hypertrophic scar fibroblasts were randomly divided into dimethyl sulfone group, shikonin group, control group, si-NC group, shikonin+ si-NC group and shikonin+si-PTENC group, western blot were used to detect the expression of PTEN and collagen-related proteins (Col1, Col3 and α-SMA）at protein levels, respectively. Results Compared with normal skin, PTEN was lower expressed in hypertrophic scar (P < 0.05). shikonin was dissolved in dimethyl sulfone to make drug solution at IC50 concentration (13.46 μmol/L), which was used for human hypertrophic scar fibroblasts cell culture for 24 hours, shikonin down-regulated the expression levels of Col1, Col3 and α-SMA in human hypertrophic scar fibroblasts, while up-regulated the expression of PTEN (P < 0.05). Transfected with si-PTEN, the expressions of Col1, Col3 and α-SMA in human hypertrophic scar fibroblasts were increased compared with the si-NC group (P < 0.05). After co-transfection with shikonin + si-PTEN, the expressions of Col1, Col3 and α-SMA in human hypertrophic scar fibroblasts were increased compared with shikonin + si-NC group (P < 0.05). Conclusion Shikonin can inhibit collagen deposition in hypertrophic scar fibroblasts by up-regulating PTEN expression. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的 从 PTEN 角度探讨紫草素对人增生性瘢痕成纤维细胞胶原沉积的影响。方法 苏木 精-伊红染色 (HE 染色) 和马松染色 (Masson 染色) 鉴定增生性瘢痕组织, 免疫荧光鉴定人增生性瘢痕成纤 维细胞 (HSFBs); Western blot 检测人增生性瘢痕 (HS) 和同一个体正常皮肤 (NS) 组织中 PTEN 表达水平; 将 HSFBs 随机分组为：DMSO 组、shikonin 组、control 组、si-NC 组、si-PTEN 组、shikonin+si-NC 组 和 shikonin+ si-PTEN 组; Western blot 检测各组中 PTEN 和胶原相关蛋白 (Col1、Col3、α⁃SMA) 的表达。结果 与正 常皮肤组织相比, PTEN 在增生性瘢痕组织中呈低表达 (P < 0.05); 用 IC50 浓度 (13.46 μmol/L) 的紫草素干 预人增生性瘢痕成纤维细胞 24 h 后, 人增生性瘢痕成纤维细胞中 Col1、Col3 和 α-SMA 的表达降低 (P < 0.05), 而 PTEN 的表达升高 (P < 0.05); 转染 si-PTEN 后, 与 si-NC 组相比, 人增生性瘢痕成纤维细胞中 Col1, Col3 和 α⁃SMA 的表达升高 (P < 0.05); 共转染 shikonin + si-PTEN 后, 与 shikonin +si-NC 组相比, 人增生性瘢 痕成纤维细胞中 Col1、ol3 和 α⁃SMA 的表达升高 (P < 0.05)。结论 紫草素可通过上调 PTEN 的表达抑制 增生性瘢痕成纤维细胞的胶原沉积。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
