Celecoxib Treatment Improves Neurologic Deficit and Reduces Selective Neuronal Loss and Glial Response in Rats after Transient Middle Cerebral Artery Occlusion
| العنوان: | Celecoxib Treatment Improves Neurologic Deficit and Reduces Selective Neuronal Loss and Glial Response in Rats after Transient Middle Cerebral Artery Occlusion |
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| المؤلفون: | Enrique Font-Belmonte, Diego Pérez-Rodríguez, Arsenio Fernández-López, María Santos-Galdiano, Irene F. Ugidos, Carlos César Pérez-García, Berta Anuncibay-Soto |
| المصدر: | Journal of Pharmacology and Experimental Therapeutics. 367:528-542 |
| بيانات النشر: | American Society for Pharmacology & Experimental Therapeutics (ASPET), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Pathology, medicine.medical_specialty, Ischemia, Infarction, Neuroprotection, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, medicine, Animals, Middle cerebral artery occlusion, Neurons, Pharmacology, Microglia, business.industry, Penumbra, Infarction, Middle Cerebral Artery, medicine.disease, Rats, Stroke, Disease Models, Animal, Neuroprotective Agents, 030104 developmental biology, medicine.anatomical_structure, nervous system, Celecoxib, Astrocytes, Reperfusion Injury, Molecular Medicine, Nervous System Diseases, business, Neuroglia, 030217 neurology & neurosurgery, Immunostaining, medicine.drug |
| الوصف: | Areas of selective neuronal loss (SNL) represent the first morphologic signs of damage in the penumbra region and are considered putative targets for ischemic stroke therapy. We performed a novel assessment of measuring the effects of the anti-inflammatory agent celecoxib by analyzing simultaneously the different neural populations (neurons, astrocytes, and microglia cells) in SNL and non-SNL areas. Rats were subjected to 1 hour of middle cerebral artery occlusion (MCAO) and treated with celecoxib 1 and 24 hours after ischemia. Infarct volume measurements and triple immunostaining of neurons (neuronal nuclear antigen), microglia (ionized calcium-binding adaptor molecule 1), and astroglia were performed after 12 and 48 hours of reperfusion. Motor response was tested by standard behavioral assays at 3, 12, 24, and 48 hours. Confocal analysis revealed that the percentage of SNL areas, microglia densities, and glial activation increased at 48 hours of reperfusion. Celecoxib treatment improved the neurologic deficit, reduced the infarct volume by 50% after 48 hours of reperfusion, and resulted in a reduced percentage of SNL areas and microglia and astroglia reactivity after 48 hours of reperfusion. This study proves, for the first time, that celecoxib presents postischemic neuroprotective effects in a transient MCAO model, prevents or delays the presence of SNL areas, and reduces glial activation. |
| تدمد: | 1521-0103 0022-3565 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5d2030d2bf46a15ad14c90be3d4706dTest https://doi.org/10.1124/jpet.118.251264Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e5d2030d2bf46a15ad14c90be3d4706d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15210103 00223565 |
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