Determination of l-norvaline and l-tryptophan in dietary supplements by nano-LC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column
| العنوان: | Determination of l-norvaline and l-tryptophan in dietary supplements by nano-LC using an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-silica hybrid monolithic column |
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| المؤلفون: | Zhengjin Jiang, Elena Sánchez-López, Qiqin Wang, Dongsheng Xu, María Luisa Marina |
| المصدر: | Journal of Pharmaceutical Analysis Journal of Pharmaceutical Analysis, Vol 10, Iss 1, Pp 70-77 (2020) |
| بيانات النشر: | Elsevier BV, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 9-Fluorenylmethoxycarbonyl, Monolithic HPLC column, 9-fluorenylmethoxycarbonyl, Pharmaceutical Science, 02 engineering and technology, Pharmacy, 01 natural sciences, Article, Analytical Chemistry, chemistry.chemical_compound, Drug Discovery, Electrochemistry, Nano-LC, Dihydroquinidine, Quinidine-silica hybrid monolithic column, Spectroscopy, chemistry.chemical_classification, Detection limit, Chromatography, lcsh:RM1-950, 010401 analytical chemistry, Tryptophan, 021001 nanoscience & nanotechnology, Chiral separation, 0104 chemical sciences, Amino acid, lcsh:Therapeutics. Pharmacology, chemistry, Amino acids, Enantiomer, Norvaline, 0210 nano-technology |
| الوصف: | An analytical methodology based on an O-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine (MQD)-silica hybrid monolithic column was developed for the enantioseparation of 9-fluorenylmethoxycarbonyl (FMOC) derivatized amino acids by nano-liquid chromatography. The mobile phase was optimized including the apparent pH, content of ACN, and concentration of the buffer to obtain a satisfactory enantioresolution performance. 27 FMOC derivatized amino acids including 19 protein and 8 non-protein amino acids were tested, and 19 out of them were enantiomerically discriminated obtaining baseline separation for 11 of them. Analytical characteristics of the method were evaluated for norvaline and tryptophan in terms of linearity, precision, accuracy, limits of detection (LOD) and quantitation (LOQ) showing good performance to be applied to the enantiomeric determination of these amino acids in dietary supplements. LOD and LOQ values were 9.3 and 31 μM for norvaline enantiomers and 7.5 and 25 μM for tryptophan enantiomers, respectively. The contents of d-norvaline and d-tryptophan were below their respective LODs in all the analyzed samples. Quantitation of l-tryptophan and l-norvaline showed good agreement with the labeled contents except for one sample which did not show presence of l-norvaline, contrary to the label indication. Graphical abstract Image 1 Highlights • A method was developed for the enantiomeric separation of amino acids by nano-LC. • A novel quinidine-silica hybrid monolith was employed as chiral column. • 19 protein and non-protein FMOC-amino acids were enantiomerically discriminated. • Analytical characteristics of the developed method were evaluated. • Norvaline and tryptophan were enantiomerically determined in dietary supplements. |
| تدمد: | 2095-1779 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87a293c0bdee753cc50c7a8d04307a5eTest https://doi.org/10.1016/j.jpha.2019.10.001Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....87a293c0bdee753cc50c7a8d04307a5e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20951779 |
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