دورية
Characterization of Prevotella intermediaand Prevotella nigrescensIsolates From Periodontic and Endodontic Infections
| العنوان: | Characterization of Prevotella intermediaand Prevotella nigrescensIsolates From Periodontic and Endodontic Infections |
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| المؤلفون: | Gharbia, Saheer E., Haapasalo, Markus, Shah, Haroun N., Kotiranta, Anja, Lounatmaa, Kari, Pearce, Michelle A., Devine, Deirdre A. |
| المصدر: | Journal of Periodontology; January 1994, Vol. 65 Issue: 1 p56-61, 6p |
| مستخلص: | The occurrence and surface propertiesof Prevotella intermediaand P. nigrescensin healthy sites and in periodontic and endodontic infections were studied among 73 strains, tentatively identified as P. intermedia. Fifteen strains were from necrotic root canal infections, 41 were from periodontal samples, and 17 isolates were obtained from healthy gingival sites. Identification of isolates as either P. intermediaor P. nigrescenswas based on differences in malate and glutamate dehydrogenase electrophoretic mobilities which allowed unambiguous separation of P. intermediaand P. nigrescens. The majority of strains from periodontal samples were P. intermedia(29 of 41 strains). In endodontic samples only 4 out of 15 isolates were P. intermedia, while all except 1 of 17 strains from healthy gingival sites were identified as P. nigrescens. SDS‐PAGE of whole cell proteins revealed 31 and 38 kDa proteins in P. nigrescenswhich were not detected in P. intermedia. Surface biotinylation of cells, followed by Western blotting and detection by alkaline phosphatase conjugated extravidin, showed strong staining of the 31 kDa protein in P. nigrescensindicating that this protein is located on the surface of the cell. Corresponding staining was not seen in P. intermedia. Fimbria‐like projections were observed using electron microscopy of negatively‐stained cells of P. nigrescens. The results show that P. intermediaand P. nigrescensmay have different site specificities and surface properties and thus emphasize the need for accurate identification of these two species for the evaluation of their role in the pathogenesis of oral infections. J Periodontol 1994;65:56–61. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00223492 19433670 |
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| DOI: | 10.1902/jop.1994.65.1.56 |