Islet neogenesis-associated protein-related pentadecapeptide improves the function of allograft after islets transplantation
| العنوان: | Islet neogenesis-associated protein-related pentadecapeptide improves the function of allograft after islets transplantation |
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| المؤلفون: | Tao Yang, Hongwen Zhou, Heng Chen, Min Sun, Xiaozhu Yu, Yun Wang, Kuanfeng Xu, Yong Gu, Mei Zhang, Yun Cai, Xinyu Xu |
| المصدر: | Journal of Pediatric Endocrinology and Metabolism. |
| بيانات النشر: | Walter de Gruyter GmbH, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | endocrine system, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Islets of Langerhans Transplantation, Pancreatitis-Associated Proteins, Diabetes Mellitus, Experimental, Immunoenzyme Techniques, Islets of Langerhans, Endocrinology, Renal capsule, Antigens, Neoplasm, Internal medicine, Insulin Secretion, Biomarkers, Tumor, medicine, Animals, Insulin, Lectins, C-Type, RNA, Messenger, Rats, Wistar, Protein kinase B, geography, Islet cell transplantation, geography.geographical_feature_category, Reverse Transcriptase Polymerase Chain Reaction, business.industry, Graft Survival, Nestin, Allografts, Islet, Peptide Fragments, Rats, Transplantation, Glucose, medicine.anatomical_structure, Pediatrics, Perinatology and Child Health, Cytokines, PDX1, business |
| الوصف: | OBJECTIVE To investigate the protective effects of a pentadecapeptide of islet neogenesis-associated protein (INGAP-PP) on transplanted islets function. METHODS Islets were cultured in RPMI 1640 with or without INGAP-PP (10 μg/mL). After 24 h, the viability of the islets and glucose-stimulated insulin secretion (GSIS) were measured. The expression of genes B cell lymphoma/lewkmia2 (Bcl2) and protein kinase B (Akt) were detected by RT-PCR assay. Healthy rats transplanted with islets under the renal capsule were injected with INGAP-PP or saline in in the abdominal cavity. One week later, the expression of insulin nestin pancreatic (nestin) and duodenal homeobox 1 (Pdx1) and proliferating cell nuclear antigen (PCNA) in the transplanted islets were observed by immunohistochemistry. After that they were transplanted to the renal capsule of diabetic rats. RESULTS 1. The amount of insulin released was increased in co-cultured group in concentration of 16.7 mmol/L glucose, which was (185.00±20.01 μU/mL) vs. (58.67±17.03 μU/mL). Gene expression of Bcl2 (0.61±0.22 vs. 0.50±0.21) and Akt (1.12±0.19 vs. 0.94±0.16) in the co-cultured group were increased compared with that of the control group. Islets viability in the co-cultured group (683.9±7.08) was higher than that of control group (547.9±8.02). The stimulating index (SI) of the co-cultured group was also higher than that of the control group. 2. The group of islets under the renal capsule which were co-cultured and injected with INGAP-PP had the more nestin expression in the islets. CONCLUSIONS The function of islet can be protected by the INGAP-PP, which will promote the viability, differentiation and regeneration of islet before transplantation. And it will be beneficial for the function of allograft after islets transplantation. |
| تدمد: | 2191-0251 0334-018X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02c037c2e41bce2dbbc0cbef02a19882Test https://doi.org/10.1515/jpem-2014-0127Test |
| رقم الانضمام: | edsair.doi.dedup.....02c037c2e41bce2dbbc0cbef02a19882 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21910251 0334018X |
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