المؤلفون: T.A. Veliksar, G.I. Drozhzhina, I.M. Mikheitseva, S.G. Kolomiichuk

المصدر: Journal of Ophthalmology, Iss 5, Pp 3-14 (2018)

مصطلحات موضوعية: neurotrophic keratoconjunctivitis, morpholinium-methyl-triazolyl-thioacetate (thiotriazolin), lactate dehydrogenase, glucose-6-phosphate dehydrogenase, acid phosphatase, succine dehydrogenase, glutathione, malondialdehyde, diene conjugates, Internal medicine, RC31-1245

الوصف: Background: Vision correction with contact lenses offers some clear advantages as compared to correction with spectacles, but has some negative effect on the eye, too. Individuals wearing soft contact lenses (SCL) for years may develop neurotrophic keratoconjunctivitis that requires a long-term and mostly symptomatic treatment. Purpose: To compare the efficacy of different routes of morpholinium-methyl-triazolyl-thioacetate (thiotriazolin) administration for neurotrophic keratoconjunctivitis in patients wearing silicone hydrogel contact lenses for different periods of time. Materials and Methods: Two hundred and seventeen eyes of 112 patients with low or moderate myopia were examined and treated. These included 65 eyes of 33 patients wearing spectacles, and 159 eyes of 79 patients wearing SCL for long periods of time. SCL wearers were divided into subgroups depending on the treatment regimen and categorized depending on duration of SCL wear. Lactate dehydrogenase, glucose-6-phosphate dehydrogenase, succine dehydrogenase, and acid phosphatase activities in the tear film, and tear contents of glutathione, malondialdehyde and diene conjugates were examined. Results and Conclusions: Treatment with thiotriazolin statistically significantly decreased lipid peroxidation activity levels, with a reduced disruption of ocular surface membranes, and protected the ocular surface from oxidative stress.

وصف الملف: electronic resource

العلاقة: https://www.ozhurnal.com/en/archive/2018/5/1-fulltextTest; https://doaj.org/toc/2412-8740Test

الوصول الحر: https://doaj.org/article/61683dd4c3c8447882249c8bfbc803dcTest

المؤلفون: T.A. Veliksar, N.F. Leus, T.B. Gaydamaka, I.N. Mikheitseva, G.I. Drozhzhyna, S.G. Kolomiichuk

المصدر: Journal of Ophthalmology, Iss 6, Pp 7-10 (2017)

مصطلحات موضوعية: cornea, contact lens, lactate dehydrogenase, glucose-6-phosphate dehydrogenase, acid phosphatase, Internal medicine, RC31-1245

الوصف: Introduction. Contact lenses are increasingly used worldwide for correcting refraction disorders. However, contact lenses can destroy a biochemical composition of the tear. Purpose. To determine the effect of silicone hydrogel contact lenses on the stability of cell membranes and membranes of subcellular structures in the corneal epithelium through detecting marker enzymes in the tear fluid. Material and Methods. We determined the activity of lactate dehydrogenase, glucose-6-phosphate dehydrogenase, and acid phosphatase in tears of patients divided into two groups. Study group comprised 13 people (24 eyes): moderate myopia patients continuously wearing soft contact lenses. Control group consisted of 20 people (40 eyes): moderate myopia patients, spectacles wearers. Results. We revealed a significant increase in the activity of lactate dehydrogenase and glucose-6-phosphate dehydrogenase by >35% and 24%, respectively, as well as a significant increase in the activity of acid phosphatase by 22.4% in the Study group patients comparing to controls. Conclusions. Continuous soft contact lens wearing increases the lability of the corneal epithelium cell membranes. We believe that pharmacological correction of such pathologic changes can prevent the development of severe complications associated with contact lens vision correction.

وصف الملف: electronic resource

العلاقة: https://www.ozhurnal.com/en/archive/2017/6/2-fulltextTest; https://doaj.org/toc/2412-8740Test

الوصول الحر: https://doaj.org/article/fc6ad3d4c0f44eb183002245a23c1669Test

المؤلفون: G.I. Drozhzhina, M.I. Pavlovsky

المصدر: Journal of Ophthalmology, Iss 1, Pp 48-50 (2016)

مصطلحات موضوعية: hypothyroidism, cornea, conjunctiva, lacrimal fluid, lactate dehydrogenase, malate dehydrogenase, Internal medicine, RC31-1245

الوصف: Introduction. Thyroid diseases rank the second place after diabetes mellitus by disease incidence. In recent years, a large number of hypothyroidism studies have showed structural and functional abnormalities in eye tissues. Purpose. To analyze the activity of lactate dehydrogenase and malate dehydrogenase in tears, cornea and conjunctiva in experimental hypothyroidism. Material and Methods. Experimental studies were performed on rats. All the animals were divided into three groups: I – control group (14 rats), II – study group (14 rats), animals with the initial stage of hypothyroidism, and III – study group (14 rats), animals with the pronounced stage of hypothyroidism. In the tissues of the cornea, conjunctiva, and lacrimal fluid, the activity of lactate dehydrogenase and malate dehydrogenase was determined. Results. The data obtained on the reduction of the activity of lactate dehydrogenase and malate dehydrogenase evidence the deterioration of the intensity of the redox processes in tissues of the anterior eye in experimental hypothyroidism. Conclusions. In experimental hypothyroidism, the activity of LDH and MDH is reduced in the cornea by 29.2% and 23.7%, and by 34.7% and 28.7% in the conjunctiva, respectively. A significant increase in the activity of redox enzymes in the lacrimal fluid was revealed in the course of the pronounced hypothyroidism stage (LDH – by 36.3%, MDH – by 24.6%). At that, the degree of change in LDH activity depends on the stage of hypothyroidism.

وصف الملف: electronic resource

العلاقة: https://www.ozhurnal.com/en/archive/2016/1/10-fulltextTest; https://doaj.org/toc/2412-8740Test

الوصول الحر: https://doaj.org/article/41a8e780758943baa5065c2b9e805b15Test