المؤلفون: Zhian Hu, Jianxia Xia, Xiaowei Chen, Zhengping Yu, Chenghui Song, Jianning Ye

المصدر: Journal of neuroscience research. 82(5)

مصطلحات موضوعية: Patch-Clamp Techniques, Potassium Channels, Action Potentials, Prefrontal Cortex, Inhibitory postsynaptic potential, Synaptic Transmission, Cellular and Molecular Neuroscience, Orexin-A, Organ Culture Techniques, Postsynaptic potential, mental disorders, Neural Pathways, Animals, Calcium Signaling, Rats, Wistar, Prefrontal cortex, Protein kinase C, Protein Kinase C, Orexins, Phospholipase C, Dose-Response Relationship, Drug, Chemistry, Pyramidal Cells, Neuropeptides, Intracellular Signaling Peptides and Proteins, Excitatory Postsynaptic Potentials, Depolarization, Neural Inhibition, Rats, Enzyme Activation, nervous system, Animals, Newborn, Type C Phospholipases, Excitatory postsynaptic potential, Calcium Channels, Neuroscience, psychological phenomena and processes, Signal Transduction

الوصف: Hypocretins are crucial for the regulation of wakefulness by the excitatory actions on multiple subcortical arousal systems. To date, there is little information about the direct postsynaptic excitatory effects of hypocretins on the neurons in prefrontal cortex (PFC), which is important for higher cognitive functions and is correlated with level of wakefulness. In this study, we tested the excitatory effects of hypocretin-1 on acutely isolated PFC pyramidal neurons of rats and studied the possible ionic mechanisms by using whole-cell patch-clamp techniques. Puff application of hypocretin-1 caused a dose-dependent excitation. Further observations that perfusion of Ca2+-free artificial cerebrospinal fluid did not influence the depolarizing effects of hypocretin-1, in conjunction with the findings that hypocretin-1 could decrease net whole-cell K+ currents, demonstrate that the excitatory effects of hypocretin-1 on PFC neurons are mediated by the inhibition of K+ currents but not Ca2+ influx. Finally, the decrease in K+ currents induced by hypocretin-1 was abolished by a protein kinase C (PKC) inhibitor (BIS II) or a phospholipase C (PLC) inhibitor (D609), suggesting that PKC and PLC appear to be involved in mediating the inhibitory effects of hypocretin-1 on K+ currents. These results indicate that hypocretin-1 exerts a postsynaptic excitatory action on PFC neurons through the inhibition of K+ currents, which probably results from activation of PKC and PLC signaling pathways.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7344df908d8bb11d70f7f2670702d157Test
https://pubmed.ncbi.nlm.nih.gov/16247802Test

المؤلفون: Jianxia Xia, Xiaowei Chen, Chenghui Song, Jianning Ye, Zhengping Yu, Zhian Hu

المصدر: Journal of Neuroscience Research; Dec2005, Vol. 82 Issue 5, p729-736, 0p