Very-high-dose α-tocopherol supplementation increases blood pressure and causes possible adverse central nervous system effects in stroke-prone spontaneously hypertensive rats
| العنوان: | Very-high-dose α-tocopherol supplementation increases blood pressure and causes possible adverse central nervous system effects in stroke-prone spontaneously hypertensive rats |
|---|---|
| المؤلفون: | Masato Koike, Yasuo Uchiyama, Motoko Shiozaki, Masahiro Shibata, Kaori Miyamoto, Takahiro Gotow |
| المصدر: | Journal of Neuroscience Research. 87:556-566 |
| بيانات النشر: | Wiley, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, medicine.medical_treatment, Blotting, Western, alpha-Tocopherol, bcl-X Protein, Cathepsin D, Blood Pressure, Biology, Neuroprotection, Lipofuscin, Lipid peroxidation, Cellular and Molecular Neuroscience, chemistry.chemical_compound, Microscopy, Electron, Transmission, Neurofilament Proteins, Internal medicine, Glial Fibrillary Acidic Protein, medicine, Animals, Tocopherol, Neurons, Cathepsin, Glial fibrillary acidic protein, Superoxide Dismutase, Vitamin E, Brain, food and beverages, Immunohistochemistry, Rats, Stroke, Endocrinology, chemistry, Hypertension, Immunology, biology.protein, lipids (amino acids, peptides, and proteins), Lipid Peroxidation, Neuroglia |
| الوصف: | Tocopherols and tocotrienols constitute the vitamin E family. Although alpha-tocotrienol is the most neuroprotective form of vitamin E proved to be effective against stroke, alpha-tocopherol is the most abundant in nature and is used most often for disease prevention/treatment. A recent metaanalysis of human studies suggested that alpha-tocopherol supplementation increases all-cause mortality. Therefore, we investigated the effects of alpha-tocopherol ( approximately 44 mg/kg body weight; equivalent to 2,600 mg/human/day) on the central nervous system (CNS) of stroke-prone spontaneously hypertensive rats (SHRSP). SHRSP treated with high dose alpha-tocopherol had significantly higher blood pressure than untreated controls fed a basal diet that contained approximately 4 mg tocopherols/kg body weight, but neither group experienced a change in degree of lipid peroxidation in serum or CNS tissue. Biochemical/immunohistochemical analyses demonstrated that expressions of phosphorylated neurofilament H protein, glial fibrillary acidic protein and cathepsin D in the CNS tissue were significantly enhanced in alpha-tocopherol-supplemented rats, whereas expressions of SOD2 and Bcl-xL were diminished in response to alpha-tocopherol supplementation. Similarly, the frequency of cathepsin D-positive cells, corresponding mostly to microglial cells, was significantly increased in alpha-tocopherol-supplemented rats. Alpha-tocopherol supplementation also increased the number of lysosomes and lipofuscin granules in perikarya of both hippocampal pyramidal and Purkinje cells. Furthermore, alpha-tocopherol supplementation increased the frequency of glial filaments and lipofuscin granules in astrocytes and lysosomes in microglial cells that were frequently occupied with phagocytosed inclusion structures. The present results are the first to suggest that a very high dose of alpha-tocopherol supplementation increases blood pressure in SHRSP rats and influences the CNS tissue in a manner that seems adverse. |
| تدمد: | 1097-4547 0360-4012 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fcc19f85cdd606e15e41525e4f35567Test https://doi.org/10.1002/jnr.21851Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....0fcc19f85cdd606e15e41525e4f35567 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974547 03604012 |
|---|