دورية
Nuclear Factor-{kappa}B Activation in Axons and Schwann Cells in Experimental Sciatic Nerve Injury and Its Role in Modulating Axon Regeneration: Studies With Etanercept
| العنوان: | Nuclear Factor-{kappa}B Activation in Axons and Schwann Cells in Experimental Sciatic Nerve Injury and Its Role in Modulating Axon Regeneration: Studies With Etanercept |
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| المؤلفون: | Smith, Darrell, Tweed, Christopher, Fernyhough, Paul, Glazner, Gordon W. |
| المصدر: | Journal of Neuropathology and Experimental Neurology; June 2009, Vol. 68 Issue: 6 p691-691, 1p |
| مستخلص: | Early inflammatory events may inhibit functional recovery after injury in both the peripheral and central nervous systems. We investigated the role of the inflammatory tumor necrosis factor/nuclear factor-κB (NF-κB) axis on events subsequent to sciatic nerve crush injury in adult rats. Electrophoretic mobility shift assays revealed that within 6 hours after crush, NF-κB DNA-binding activity increased significantly in a 1-cm section around the crush site. By immunofluorescence staining, there was increased nuclear localization of the NF-κB subunits p50 but not p65 or c-Rel in Schwann cells but no obvious inflammatory cell infiltration. In rats injected subcutaneously with etanercept, a tumor necrosis factor receptor chimera that binds free cytokine, the injury-induced rise in NF-κB DNA-binding activity was inhibited, and nuclear localization of p50 in Schwann cells was lowered after the injury. Axonal growth 3 days after nerve crush assessed with immunofluorescence for GAP43 demonstrated that the regeneration distance of leading axons from the site of nerve crush was greater in etanercept-treated animals than in saline-treated controls. These data indicate that tumor necrosis factor mediates rapid activation of injury-induced NF-κB DNA binding in Schwann cells and that these events are associated with inhibition of postinjury axonal sprouting. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00223069 15546578 |
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| DOI: | 10.1097/NEN.0b013e3181a7c14e |