Interlaboratory Comparison of Assessments of Alzheimer Disease-Related Lesions: A Study of the BrainNet Europe Consortium
| العنوان: | Interlaboratory Comparison of Assessments of Alzheimer Disease-Related Lesions: A Study of the BrainNet Europe Consortium |
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| المؤلفون: | David Meyronet, Isidro Ferrer, Nenad Bogdanovic, Herbert Budka, Manuel B. Graeber, Nicolas Kopp, Andy King, Maria Pikkarainen, Giorgio Giaccone, Ellen Gelpi, Irina Alafuzoff, Piero Parchi, Safa Al-Sarraj, Dietmar Rudolf Thal, Matthias Preusser, Rivka Ravid, Danielle Seilhean, Istvan Bodi, Hans A. Kretzschmar, Jean Jacques Hauw, Nathalie Streichenberger, Penelope Korkolopoulou, Jeanne E. Bell, Efstratios Patsouris, Thomas Arzberger, Wolfgang Roggendorf, Gabor G. Kovacs, Wouter Kamphorst, Orso Bugiani |
| المساهمون: | Alafuzoff I., Pikkarainen M., Al-Sarraj S., Arzberger T., Bell J., Bodi I., Bogdanovic N., Budka H., Bugiani O., Ferrer I., Gelpi E., Giaccone G., Graeber M.B., Hauw J.J., Kamphorst W., King A., Kopp N., Korkolopoulou P., Kovacs G.G., Meyronet D., Parchi P., Patsouris E., Preusser M., Ravid R., Roggendorf W., Seilhean D., Streichenberger N., Thal D.R., Kretzschmar H. |
| المصدر: | Journal of Neuropathology & Experimental Neurology. 65:740-757 |
| بيانات النشر: | Oxford University Press (OUP), 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Male, Silver Staining, Pathology, medicine.medical_specialty, Biopsy, Tau protein, Plaque, Amyloid, tau Proteins, Tissue Banks, Neuropathology, Beta-amyloid, Stain, Tissue microarray, Pathology and Forensic Medicine, Cellular and Molecular Neuroscience, Alzheimer Disease, Neuropathologic diagnosi, Humans, Medicine, Hyperphosphorylated tau, Registries, Senile plaques, Aged, Aged, 80 and over, Cerebral Cortex, Interlaboratory study, Amyloid beta-Peptides, Staining and Labeling, biology, business.industry, International Agencies, Neurofibrillary Tangles, General Medicine, Middle Aged, medicine.disease, Immunohistochemistry, Staining, Europe, Neurology, biology.protein, Female, Neurology (clinical), Alzheimer's disease, business |
| الوصف: | This interlaboratory study evaluated the reproducibility of the assessments of neuritic plaques and neurofibrillary tangles (NFTs)-the hallmark lesions of Alzheimer disease-and compared the staining between the BrainNet Europe centers. To reduce the topography-related inconsistencies in assessments, we used a 2-mm tissue microarray (TMA) technique. The TMA block included 42 core samples taken from 21 paraffin blocks. The assessments were done on Bielschowsky and Gallyas silver stains using an immunohistochemical (IHC) method with antibodies directed to beta-amyloid (IHC/Aβ) and hyperphosphorylated tau (IHC/HPtau). The staining quality and the assessments differed between the participants, being most diverse with Bielschowsky (good/acceptable stain in 53% of centers) followed by Gallyas (good/acceptable stain in 57%) and IHC/Aβ (good/acceptable stain in 71%). The most uniform staining quality and assessment was obtained with the IHC/HPtau method (good/acceptable stain in 94% of centers). The neuropathologic diagnostic protocol (Consortium to Establish a Registry for Alzheimer Disease, Braak and Braak, and the National Institute of Aging and Reagan [NIA-Reagan] Institute) that was used significantly influenced the agreement, being highest with NIA-Reagan (54%) recommendations. This agreement was improved by visualization of NFTs using the IHC/HPtau method. Therefore, the IHC/HPtau methodology to visualize NFTs and neuropil threads should be considered as a method of choice in a future diagnostic protocol for Alzheimer disease. Copyright © 2006 by the American Association of Neuropathologists, Inc. |
| وصف الملف: | STAMPA |
| تدمد: | 1554-6578 0022-3069 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e76b3c032c63e4c15be024612da3d97Test https://doi.org/10.1097/01.jnen.0000229986.17548.27Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3e76b3c032c63e4c15be024612da3d97 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15546578 00223069 |
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