Interferon-γ Modulates Human Oligodendrocyte Susceptibility To Fas-Mediated Apoptosis
| العنوان: | Interferon-γ Modulates Human Oligodendrocyte Susceptibility To Fas-Mediated Apoptosis |
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| المؤلفون: | Jack P. Antel, Sandrine Pouly, Burkhard Becher, Manon Blain |
| المصدر: | Journal of Neuropathology & Experimental Neurology. 59:280-286 |
| بيانات النشر: | Oxford University Press (OUP), 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | medicine.medical_treatment, Apoptosis, Cysteine Proteinase Inhibitors, Pathology and Forensic Medicine, Interferon-gamma, Cellular and Molecular Neuroscience, medicine, Humans, Cytotoxic T cell, Interferon gamma, RNA, Messenger, fas Receptor, Cells, Cultured, L-Lactate Dehydrogenase, biology, Reverse Transcriptase Polymerase Chain Reaction, Tumor Necrosis Factor-alpha, Cell Membrane, Antibodies, Monoclonal, Drug Synergism, Myelin Basic Protein, General Medicine, Flow Cytometry, Fas receptor, Antigens, Differentiation, Caspase Inhibitors, Molecular biology, Oligodendrocyte, Nucleosomes, Up-Regulation, Myelin basic protein, Oligodendroglia, medicine.anatomical_structure, Cytokine, Neurology, Cell culture, biology.protein, Neurology (clinical), medicine.drug |
| الوصف: | Interferon gamma (IFN-gamma) has been shown to be produced within multiple sclerosis (MS) lesions by infiltrating lymphocytes; systemic administration of this cytokine induces exacerbation of the disease. The aim of the current study was to establish the contribution of IFN-gamma to oligodendrocyte (OL) injury. Our studies utilized cultured human OLs, obtained by dissociation of surgically derived non-MS adult brain tissue. Neither cell survival nor myelin basic protein (MBP) gene expression were affected after 96 hours of treatment with IFN-gamma (100 U/ml), as assessed by LDH release, nucleosome enrichment assay, and RT-PCR. Expression of the death receptor Fas (CD95, APO-1) was, however, significantly increased. Furthermore, IFN-gamma-treated OLs became susceptible to Fas-mediated apoptosis when compared with untreated cells, and were protected by pretreatment with the caspase inhibitor ZVAD. TNF-alpha augmented the IFN-gamma-induced effect. Our results thus indicate that IFN-gamma is not directly cytotoxic for human OLs in culture, but could indirectly modulate functional injury-related responses by upregulating Fas on the cell surface. |
| تدمد: | 1554-6578 0022-3069 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e3a25c9e49abceafba371a75096c556Test https://doi.org/10.1093/jnen/59.4.280Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2e3a25c9e49abceafba371a75096c556 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15546578 00223069 |
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