التفاصيل البيبلوغرافية
| العنوان: |
Involvement ofγ protein kinase C in estrogen-induced neuroprotection against focal brain ischemia through G protein-coupled estrogen receptor. |
| المؤلفون: |
Hayashi, Shigeto, Ueyama, Takehiko, Kajimoto, Taketoshi, Yagi, Keiko, Kohmura, Eiji, Saito, Naoaki |
| المصدر: |
Journal of Neurochemistry; May2005, Vol. 93 Issue 4, p883-891, 9p |
| مصطلحات موضوعية: |
ESTROGEN, ISCHEMIA, REPERFUSION, MEMBRANE proteins, PROTEIN kinase C, ESTROGEN receptors |
| مستخلص: |
The neuroprotective effects of estrogen were studied in the ischemic model mice by 90 min transient unilateral middle cerebral artery occlusion (MCAO) followed by 22.5 h reperfusion. The total infarct size in C57BL/6 female mice after MCAO and reperfusion was significantly smaller than that in male mice. Intraperitoneal injection of estrogen after the start of reperfusion significantly reduced the infarct volume in the male mice. However, no significant gender difference was found in total infarct size inγ protein kinase C (PKC)-knockoutmice, suggesting that the neuroprotective effects of estrogen are due to the activation of a specific subtype of PKC,γPKC, a neuron-specific PKC subtype, in the brain. We demonstrated that exogenous estrogen-induced neuroprotection was attenuated inγPKC-knockout mice. Immunocytochemical study showed thatγPKC was translocated to nerve fiber-like structures when observed shortly after MCAO and reperfusion. We also visualized the rapid and reversible translocation ofγPKC-GFP (green fluorescent protein)by estrogen stimulation in living CHO-K1 cells. These results suggest that the activation ofγPKC through the G-protein-coupled estrogen receptors on the plasma membrane is involved in the estrogen-induced neuroprotection against focal brain ischemia. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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