Production of Mycobacterium bovisAntigens Included in Recombinant Occlusion Bodies of Baculovirus

التفاصيل البيبلوغرافية
العنوان:	Production of Mycobacterium bovisAntigens Included in Recombinant Occlusion Bodies of Baculovirus
المؤلفون:	Villafañe, Luciana, Forrellad, Marina Andrea, López, María Gabriela, Garbaccio, Sergio, Garro, Carlos, Rocha, Rosana Valeria, Eirin, María Emilia, Singh, Mahavir, Taboga, Oscar A., Bigi, Fabiana
المصدر:	Journal of Molecular Microbiology and Biotechnology; July 2020, Vol. 29 Issue: 1-6 p83-90, 8p
مستخلص:	Bovine tuberculosis (bTB) is a disease produced by Mycobacterium bovisthat affects livestock, wild animals, and humans. The classical diagnostic method to detect bTB is measuring the response induced with the intradermal injection of purified protein derivative of M. bovis(PPDb). Another ancillary bTB test detects IFN-γ produced in whole blood upon stimulation with PPDb, protein/peptide cocktails, or individual antigens. Among the most used M. bovisantigens in IFN-γ assays are the secreted proteins ESAT-6 and CFP-10, which together with antigen Rv3615c improve the sensitivity of the test in comparison to PPDb. Protein reagents for immune stimulation are generally obtained from Escherichia coli, because this bacterium produces a high level of recombinant proteins. However, E. colirecombinant antigens are in general contaminated with lipopolysaccharides and other components that produce non-specific IFN-γ secretion in in vitro assays. In this work, we produced the relevant ESAT-6, CFP-10, and Rv3615c M. bovisantigens as fusions to the polyhedrin protein from the baculovirus AcMNPV. We obtained chimeric proteins effectively incorporated to the occlusion bodies and easily purified the recombinant polyhedra with no reactive contaminants. In an IFN-γ assay, these fusion proteins showed equivalent sensibility but better specificity than the same M. bovisproteins produced in E. coli.
قاعدة البيانات:	Supplemental Index

الوصف
تدمد:	14641801 16602412
DOI:	10.1159/000506687