Locust primary neuronal culture for the study of synaptic transmission
| العنوان: | Locust primary neuronal culture for the study of synaptic transmission |
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| المؤلفون: | Simone Meffert, Peter Bräunig, Stefan Weigel, Andreas Offenhäusser, Petra Schulte |
| المصدر: | Journal of Molecular Histology. 43:405-419 |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, Patch-Clamp Techniques, Histology, GABA Agents, Physiology, Chemical synapse, Cell Culture Techniques, Action Potentials, Grasshoppers, Neurotransmission, Biology, Synaptic Transmission, Electrical Synapses, Postsynaptic potential, medicine, Animals, Patch clamp, Electrical synapse, Motor Neurons, Cell Biology, General Medicine, Anatomy, Stimulation, Chemical, medicine.anatomical_structure, nervous system, Female, Ganglia, Neuron, Neuroscience, Non-spiking neuron |
| الوصف: | We have designed a cell culture system for thoracic neurons of adult Locusta migratoria that enables the establishment of functional synapses in vitro. Patch-clamp recordings revealed three different neuron classes. About half of the neurons (47%) had unexcitable somata with outward and no inward conductance. The other half generated either single (37%) or multiple action potentials (18%) and differed mainly in lower outward conductance. Selectively stained motor neurons were analyzed to demonstrate varied physiological properties due to culture conditions. Using paired patch clamp recordings we demonstrate directly synaptic transmission in morphologically connected neurons in vitro. Presynaptic stimulation resulted in postsynaptic potentials in 42 pairs of neurons tested, independent of the type of neuron. According to pharmacological experiments most of these synapses were either glutamatergic or GABAergic. In addition to these chemical synapses, electrical synapses were found. With the demonstration of synapse formation in cell culture of adult locust neurons, this study provides the basis for the future analysis of more defined insect neuronal circuits in culture. |
| تدمد: | 1567-2387 1567-2379 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb27264bf7bdbd0dc72a652e9a8de1b2Test https://doi.org/10.1007/s10735-012-9395-1Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....cb27264bf7bdbd0dc72a652e9a8de1b2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15672387 15672379 |
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