Development of a High Efficient ��Dual Bt-Plus�� Insecticide Using a Primary Form of an Entomopathogenic Bacterium, Xenorhabdus nematophila
العنوان: | Development of a High Efficient ��Dual Bt-Plus�� Insecticide Using a Primary Form of an Entomopathogenic Bacterium, Xenorhabdus nematophila |
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المؤلفون: | Seonghyeon Eom, Hyeonghwan Kim, Yonggyun Kim, Youngjin Park |
المصدر: | Journal of Microbiology and Biotechnology. 24:507-521 |
بيانات النشر: | Journal of Microbiology and Biotechnology, 2014. |
سنة النشر: | 2014 |
مصطلحات موضوعية: | Phase variation, Insecticides, Xenorhabdus nematophila, biology, Chemistry, Bacillus thuringiensis, Plutella, General Medicine, Spodoptera, biology.organism_classification, Survival Analysis, Applied Microbiology and Biotechnology, Xenorhabdus, Microbiology, Lepidoptera, Exigua, Animals, Eicosanoids, Bioassay, Biological Assay, Pest Control, Biological, Bacteria, Biotechnology |
الوصف: | A phase variation has been reported in an entomopathogenic bacterium, Xenorhabdus nematophila. Compared with a wild-type primary form, a secondary form usually loses several physiological and biochemical characters. This study showed that the phase variation of X. nematophila caused a significant alteration in its immunosuppressive activity and subsequent entomopathogenicity. A secondary form of X. nematophila was detected in laboratory colonies and exhibited significant differences in dye absorption and entomopathogenicity. In addition, the secondary form was different in its production of eicosanoid-biosynthesis inhibitors (EBIs) compared with the primary form of X. nematophila. Production of oxindole and phydroxypropionic acid was significantly reduced in the culture broth of the secondary form of X. nematophila. The reduced EBI production resulted in significant suppression in the inhibitory effects on cellular nodule formation and phenoloxidase activity. Culture broth of the primary form of X. nematophila enhanced the pathogenicity of Bacillus thuringiensis ( Bt) significantly more than the culture broth of the secondary form. Furthermore, this study developed a highly efficient "Dual Bt-Plus" to control both lepidopteran insect pests Plutella xylostella and Spodoptera exigua, by mixing two effective Bt strains along with the addition of potent bacterial metabolites or 100-fold concentrated X. nematophila culture broth. |
تدمد: | 1738-8872 1017-7825 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::995e1b86eef40b44bbd98c88844fdaf9Test https://doi.org/10.4014/jmb.1310.10116Test |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....995e1b86eef40b44bbd98c88844fdaf9 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 17388872 10177825 |
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