The effects of cigarettes and alcohol on intestinal microbiota in healthy men
| العنوان: | The effects of cigarettes and alcohol on intestinal microbiota in healthy men |
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| المؤلفون: | Mengjia Hu, Ying Zhang, Shujie Chen, Tao Yang, Lina Fan, Renbin Lin, Yawen Zhang, Misi Si, Luyi Chen, Jiamin He, Yadong Qi, Lan Wang |
| المصدر: | Journal of Microbiology. 58:926-937 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Male, Alcohol Drinking, Firmicutes, Physiology, Alcohol, Gut flora, Applied Microbiology and Biotechnology, Microbiology, Group A, Cigarette Smoking, Feces, Young Adult, 03 medical and health sciences, chemistry.chemical_compound, medicine, Humans, Aged, 030304 developmental biology, 0303 health sciences, Bacteria, biology, 030306 microbiology, business.industry, General Medicine, Middle Aged, Fatty Acids, Volatile, biology.organism_classification, medicine.disease, Healthy Volunteers, Gastrointestinal Microbiome, chemistry, Bacteroides, business, Dysbiosis, Ruminococcaceae |
| الوصف: | Human intestinal microbiota is affected by the exogenous microenvironment. This study aimed to determine the effects of cigarettes and alcohol on the gut microbiota of healthy men. In total, 116 healthy male subjects were enrolled and divided into four groups: non-smoking and non-drinking (Group A), smoking only (Group B), drinking only (Group C), and smoking and drinking combined (Group D). Fecal samples were collected and sequenced using 16S rRNA to analyze the microbial composition. Short-chain fatty acid (SCFAs) levels in feces were determined by gas chromatography. We found that cigarette and alcohol consumptions can alter overall composition of gut microbiota in healthy men. The relative abundances of phylum Bacteroidetes and Firmicutes and more than 40 genera were changed with cigarette and alcohol consumptions. SCFAs decreased with smoking and alcohol consumption. Multivariate analysis indicated that when compared with group A, group B/C/D had higher Bacteroides, and lower Phascolarctobacterium, Ruminococcaceae_UCG-002, Ruminococcaceae_UCG-003, and Ruminiclostridium_9 regardless of BMI and age. Additionally, the abundance of Bacteroides was positively correlated with the smoking pack-year (r = 0.207, p < 0.05), the abundance of predicted pathway of bacterial toxins (r = 0.3672, p < 0.001) and the level of carcinoembryonic antigen in host (r = 0.318, p < 0.01). Group D shared similar microbial construction with group B, but exerted differences far from group C with lower abundance of Haemophilus. These results demonstrated that cigarette and alcohol consumption separately affected the intestinal microbiota and function in healthy men; furthermore, the co-occurrence of cigarette and alcohol didn't exacerbate the dysbiosis and cigarette played the predominated role on the alteration. |
| تدمد: | 1976-3794 1225-8873 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b003dd53cc743411c81cd2fc49517dcfTest https://doi.org/10.1007/s12275-020-0006-7Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....b003dd53cc743411c81cd2fc49517dcf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19763794 12258873 |
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