دورية
Choice of Laboratory Tissue Homogenizers Matters When Recovering Nucleic Acid From Medically Important Ticks
| العنوان: | Choice of Laboratory Tissue Homogenizers Matters When Recovering Nucleic Acid From Medically Important Ticks |
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| المؤلفون: | Jones, Amanda M, Van de Wyngaerde, Marshall T, Machtinger, Erika T, Rajotte, Edwin G, Baker, Thomas C |
| المصدر: | Journal of Medical Entomology; July 2020, Vol. 57 Issue: 4 p1221-1227, 7p |
| مستخلص: | Ticks can vector and transmit many pathogens and pose a serious human health threat throughout the world. After collection, many diagnostic laboratories must mechanically disrupt tick specimens for diagnostic testing and research purposes, but few studies have evaluated how well-commercial tissue homogenizers perform this task. We evaluated four commercially available tissue homogenizers: The Bead Ruptor 24 Elite, the Bullet Blender Storm, the gentleMACS Dissociator, and the Precellys 24. We quantitatively compared maceration level, nucleic acid quality, quantity, amplification, and DNA shearing to determine which machines performed the best. The Bead Ruptor 24 Elite had the highest overall score when disrupting a single, uninfected adult Amblyomma americanum(Linnaeus) (Ixodida: Ixodidae) and performed well in follow-on tests including disrupting individual juvenile samples and detecting pathogens from infected samples. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00222585 19382928 |
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| DOI: | 10.1093/jme/tjaa006 |