المؤلفون: Wang, Qian, Guo, Wen-Bin, Pan, Yu-Sheng, Jiang, Bao-Gui, Du, Chun-Hong, Que, Teng-Cheng, Zhan, Lin, Wu, Jia-Hong, Yu, Ming-Hui, Cui, Xiao-Ming, Zhao, Lin, Xu, Da-Li, Xia, Luo-Yuan, Ye, Run-Ze, Li, Jie, Li, Lian-Feng, Wei, Wei, Zhou, Yu-Hao, Jiang, Jia-Fu, Jia, Na, Cao, Wu-Chun

المصدر: Journal of Medical Entomology; May 2021, Vol. 58 Issue: 3 p1363-1369, 7p

مستخلص: Spotted fever group rickettsiae, mainly maintained and transmitted by ticks, are important etiological agents of (re)emerging zoonotic diseases worldwide. It is of great significance to investigate spotted fever group rickettsiae in ticks in different areas for the prevention and control of rickettsioses. In this study, a total of 305 ticks were collected from wild and domestic animals in Chongqing, Guizhou, Yunnan, and Guangxi provinces of southwestern China during 2017–2019 and examined for the presence of spotted fever group rickettsiae by PCR with primers targeting the partial gltA, ompA, rrs, and htrAgenes. Results showed that two spotted fever group rickettsiae species, including the pathogenic CandidatusRickettsia jingxinensis (Rickettsiales: Rickettsiaceae) and a potential novel species Rickettsiasp. sw (Rickettsiales: Rickettsiaceae), were identified. The Ca.R. jingxinensis sequences were recovered from Rhipicephalus microplus(Ixodida: Ixodidae) and Haemaphysalis longicornis(Ixodida: Ixodidae) ticks and phylogenetically clustered with previous Ca.R. jingxinensis, Ca.R. longicornii (Rickettsiales: Rickettsiaceae), and Rickettsiasp. XY118 (Rickettsiales: Rickettsiaceae) strains. Rickettsiasp. sw was detected in Amblyomma geoemydae(Ixodida: Ixodidae) and Rh. microplus. Interestingly, as far as we know, this was the first report of Rickettsia(Rickettsiales: Rickettsiaceae) in A. geoemydae. Phylogenetic analyses indicated that this potential novel species was closely related to R. aeschlimannii(Rickettsiales: Rickettsiaceae) with gltAand ompAgenes and grouped in a cluster composed of R. montanensis(Rickettsiales: Rickettsiaceae), R. raoultii(Rickettsiales: Rickettsiaceae), R. aeschlimannii, R. massiliae(Rickettsiales: Rickettsiaceae), and R. rhipicephali(Rickettsiales: Rickettsiaceae) with htrA, while formed a separate clade with rrs. The pathogenicity of Rickettsiasp. sw should be further confirmed. These results expand the knowledge of the geographical distribution and vector distribution of spotted fever group rickettsiae in China and are useful for assessing the potential public health risk.

المؤلفون: Li, Lian-Feng, Wei, Ran, Liu, Hong-Bo, Jiang, Bao-Gui, Cui, Xiao-Ming, Wei, Wei, Yuan, Ting-Ting, Wang, Qian, Zhao, Lin, Xia, Luo-Yuan, Li, Jie, Jiang, Jia-Fu, Jia, Na, Hu, Yan-Ling

المصدر: Journal of Medical Entomology; July 2020, Vol. 57 Issue: 4 p1270-1276, 7p

مستخلص: The importance of ticks in veterinary and medical science has received much attention. The dominant tick species in northeastern China, Ixodes persulcatustick can transmit various pathogens to humans and animals and there are some studies on the microbiome composition of this tick. Our study characterized the bacterial communities in I. persulcatusby 16S amplicon pyrosequencing and described the differences of microorganisms in male and female tick and assessed the variation of microorganisms in the development stages in northeastern China. We mainly found the following bacteria genera: Pseudomonas(Pseudomonadales: Pseudomonadaceae), Citrobacter(Enterobacteriales: Enterobacteriaceae), and Rickettsia(Rickettsiales: Rickettsiaceae). Rickettsiais common and harmful pathogen transmitted by ticks. Meanwhile, we found there were differences between male and female tick of microbiomes, and the diversity of microbiome increased from engorged female ticks to eggs, but decreased when the eggs were molting into larvae. Our data showed that male ticks exhibited greater microbial diversity than female I. persulcatustick and larvae presented with a different bacterial community compared to engorged female tick and hatched eggs. These findings may be useful for further understanding the interaction between I. persulcatusand microbiome biology.

المؤلفون: Li, Lian-Feng^1,2, Wei, Ran^2,3, Liu, Hong-Bo^2,4, Jiang, Bao-Gui², Cui, Xiao-Ming², Wei, Wei^2,5, Yuan, Ting-Ting^2,6, Wang, Qian^2,7, Zhao, Lin^2,7, Xia, Luo-Yuan², Li, Jie², Jiang, Jia-Fu², Jia, Na², Hu, Yan-Ling^1,5,1

المصدر: Journal of Medical Entomology 57(4):1270-1276. 2020