دورية أكاديمية
Evaluation of a new molecular test for the detection of SARS-CoV-2 nucleic acid in salivary samples
| العنوان: | Evaluation of a new molecular test for the detection of SARS-CoV-2 nucleic acid in salivary samples |
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| المؤلفون: | Talli Ilaria, Padoan Andrea, Moz Stefania, Navaglia Filippo, Plebani Mario, Basso Daniela |
| المصدر: | Journal of Medical Biochemistry, Vol 42, Iss 4, Pp 658-664 (2023) |
| بيانات النشر: | Society of Medical Biochemists of Serbia, Belgrade, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biochemistry |
| مصطلحات موضوعية: | covid-19, molecular testing, nucleic acid amplification, salivary samples, sars-cov-2, Biochemistry, QD415-436 |
| الوصف: | Background: Molecular testing is considered the gold standard for the detection of SARS-CoV-2. This study aimed to compare the performance of the P742H SARS-CoV-2 Nucleic Acid Multiplex Detection Kit in salivary samples, with respect to the 732HF Novel Coronavirus (2019-nCoV) Nucleic Acid Detection Kit and the TaqPath COVID-19 CEIVD RT-PCR Kit, used at University-Hospital of Padova, Italy. Methods: One hundred twenty-four salivary samples selfcollected by healthcare workers (HCW) during the screening program at University-Hospital of Padova, Italy, from Oct to Nov 2022, were included in the study. RNA extraction was performed by Viral DNA and RNA Extraction Kit (Technogenetics, Lodi, Italy) and amplification by P742H and 732HF (Technogenetics, Lodi, Italy). RNA was extracted using MagNa Pure 96 DNA and Viral NA Small Volume Kit (Roche, Switzerland) for TaqPath analysis (Thermo Fisher Scientific, USA). Results: 94 samples were positive at P742H, while 30 were negative; for 732HF, 96 samples were positive, while 28 were negative, with an overall agreement of 97.5% (Cohen's = 0.930, p < 0.001). TaqPath gave 95 positive samples, and 29 negative results, with an overall agreement of 100% (Cohen's = 1.0, p < 0.001) with respect to P742H, and 97.5% (Cohen's = 0.931, p < 0.001) with respect to 732HF. Comparing cycle threshold (Ct) between the P742H and 732HF, no statistically significant differences were found (p = n.s.). Conclusion: The P742H method proved better performances than 732HF for salivary samples, both presenting the same amplification time. In addition, P742H results were comparable to those obtained through the highthroughput method TaqPath. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1452-8258 1452-8266 |
| العلاقة: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2023/1452-82582304658T.pdfTest; https://doaj.org/toc/1452-8258Test; https://doaj.org/toc/1452-8266Test |
| DOI: | 10.5937/jomb0-43822 |
| الوصول الحر: | https://doaj.org/article/0f139673ca524ea7be145bfcae1f6a62Test |
| رقم الانضمام: | edsdoj.0f139673ca524ea7be145bfcae1f6a62 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14528258 14528266 |
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| DOI: | 10.5937/jomb0-43822 |