التفاصيل البيبلوغرافية
| العنوان: |
Development and application of a UHPLC-MS/MS method for the simultaneous determination of 17 steroidal hormones in equine serum |
| المؤلفون: |
Fulvio Laus, Riccardo Petrelli, Giovanni Caprioli, Manuela Cortese, Michele Genangeli, Stefano Sartori, Mara Matteucci, Sauro Vittori, Gianni Sagratini, Massimo Ricciutelli |
| المصدر: |
Journal of Mass Spectrometry. 52:22-29 |
| بيانات النشر: |
Wiley, 2016. |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
0301 basic medicine, Androsterone, Chromatography, 010401 analytical chemistry, Dehydroepiandrosterone, Estrone, 01 natural sciences, 0104 chemical sciences, Matrix (chemical analysis), 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, Dehydroepiandrosterone sulfate, chemistry, Dihydrotestosterone, medicine, Pregnenolone, Spectroscopy, Testosterone, medicine.drug |
| الوصف: |
A new, fast and simple analytical method that is able to identify and quantify simultaneously 17 steroid hormones and metabolites (pregnenolone, 17-OH-pregnenolone, progesterone, 17-OH-progesterone, androsterone, androstenedione, dehydroepiandrosterone, dehydroepiandrosterone sulfate, testosterone, cortisol, corticosterone, aldosterone, 11-deoxycortisol, 11-deoxycorticosterone, dihydrotestosterone, estrone and estradiol) has been developed in equine serum using the ultra-high-performance liquid chromatography-tandem mass spectrometry technique. A total of 400 µl of sample was deproteinized with 1000 µl of acetonitrile, evaporated, restored with 50 µl of a solution of 25% methanol and injected in ultra-high-performance liquid chromatography-tandem mass spectrometry triple quadrupole. The recovery percentage obtained by spiking the matrix at two different concentrations with a standard mixture of steroid hormones was in all cases higher than 85.60% and with the percentage of coefficient of variation lower than 8.37%. The range of the correlation coefficients of the calibration curves of the analyzed compounds was 0.9922-0.9986, and the limits of detection and limits of quantification were in the range of 0.002-2 and 0.0055-5.5 ng ml-1 , respectively. The detected limit of quantification for testosterone (i.e. 50 pg ml-1 ) is twofold lower with respect to its threshold admitted in geldings plasma (100 pg ml-1 free testosterone). The high sensitivity and the quantitative aspect of the method permitted to detect most of the steroids in equine serum. Once validated, the method was used to quantify 17 steroid hormones in mare, stallion and gelding serum samples. The main steroids detected were corticosterone (range 37.25-51.26 ng ml-1 ) and cortisol (range 32.57-52.24 ng ml-1 ), followed by 17-OH-pregnenolone, dihydrotestosterone and pregnenolone. Copyright © 2016 John Wiley & Sons, Ltd. |
| تدمد: |
1076-5174 |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_________::ba52010d8f4b0a085c347ad95f8d2ee2Test
https://doi.org/10.1002/jms.3896Test |
| حقوق: |
OPEN |
| رقم الانضمام: |
edsair.doi...........ba52010d8f4b0a085c347ad95f8d2ee2 |
| قاعدة البيانات: |
OpenAIRE |
