Evaluation of the Performance of Direct Susceptibility Test by VITEK-2 from Positively Flagged Blood Culture Broth for Gram-Negative Bacilli
| العنوان: | Evaluation of the Performance of Direct Susceptibility Test by VITEK-2 from Positively Flagged Blood Culture Broth for Gram-Negative Bacilli |
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| المؤلفون: | Apurba Sankar Sastry, Sarumathi Dhandapani, Suman Susan Prakash, Deepashree Rajshekar, D Kavipriya |
| المصدر: | Journal of Laboratory Physicians, Vol 13, Iss 04, Pp 374-379 (2021) Journal of Laboratory Physicians |
| بيانات النشر: | Georg Thieme Verlag KG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, bloodstream infections, Klebsiella pneumoniae, 030106 microbiology, medicine.disease_cause, law.invention, Microbiology, 03 medical and health sciences, 0302 clinical medicine, law, categorical agreement, medicine, Blood culture, 030212 general & internal medicine, antimicrobial susceptibility testing, biology, medicine.diagnostic_test, Pseudomonas aeruginosa, business.industry, Gram negative bacilli, essential agreement, biology.organism_classification, Antimicrobial, Enterobacteriaceae, Acinetobacter baumannii, Gram staining, vitek-2, Medicine, Original Article, business, nonfermenters, enterobacteriaceae |
| الوصف: | Background Timely initiation of antimicrobial therapy in patients with blood stream infection is absolutely necessary to reduce mortality and morbidity. Most clinical microbiology laboratories use conventional methods for identification and antimicrobial susceptibility testing (AST) that involve biochemical methods for identification followed by AST by disk diffusion. The aim of the current study is to assess the various errors associated with direct susceptibility testing done from blood culture broth using automated AST system-Vitek-2 compact compared with the reference method of AST done from bacterial colonies. Materials and Methods The study was conducted in a tertiary care public sector 2,200-bedded hospital in South India for a period of 6 months. The study involved positively flagged blood culture bottles that yielded single morphotype of Gram-negative organism by Gram stain. A total of 120 bacterial isolates were collected that consisted of consecutively obtained first 60 isolates of Enterobacteriaceae family (30 Escherichia coli and 30 Klebsiella pneumoniae) and consecutively obtained first 60 nonfermenters (30 Pseudomonas aeruginosa and 30 Acinetobacter baumannii). Vitek-2 AST was done from these 120 blood culture broth, following the protocol by Biomerieux, and results were obtained. Then, Vitek-2 was done from colonies (reference method) using appropriate panel for Enterobacteriaceae and nonfermenters, and results were obtained. Both the results were compared. Results Nonfermenters showed a better categorical agreement of 97.6%, as compared to Enterobacteriaceae, which showed 97%. Among Enterobacteriaceae, both E. coli and K. pneumoniae showed categorical agreement of 97% each. Conclusion The procedure of AST directly from blood culture broth represents a simple and effective technique that can reduce the turnaround time by 24 hours, which in turn benefits the clinician in appropriate utilization of antimicrobials for better patient care. |
| تدمد: | 0974-7826 0974-2727 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa7d02a8d423b3954efad7319bdcf62dTest https://doi.org/10.1055/s-0041-1732489Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....aa7d02a8d423b3954efad7319bdcf62d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09747826 09742727 |
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