Fully automated production of the fibroblast activation protein radiotracer [ 18 F]FAPI‐74
| العنوان: | Fully automated production of the fibroblast activation protein radiotracer [ 18 F]FAPI‐74 |
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| المؤلفون: | Lovisa Bylund, Kenneth Dahl, Thuy A. Tran, Erik Samén, Emma Jussing, Mohammad Mahdi Moein |
| المصدر: | Journal of Labelled Compounds and Radiopharmaceuticals. 64:346-352 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Aluminum fluoride, 010405 organic chemistry, Chemistry, Organic Chemistry, Radiosynthesis, Radiochemistry, Pet imaging, 01 natural sciences, Biochemistry, 030218 nuclear medicine & medical imaging, 0104 chemical sciences, Analytical Chemistry, 03 medical and health sciences, 0302 clinical medicine, Fully automated, Fibroblast activation protein, alpha, Yield (chemistry), Drug Discovery, Radiology, Nuclear Medicine and imaging, Spectroscopy |
| الوصف: | We report herein an efficient and fully automated protocol for the radiosynthesis of [18 F]FAPI-74, a new positron emission tomography (PET) radiopharmaceutical for in vivo detection of the fibroblast activation protein. [18 F]FAPI-74 was synthesized via a rapid [18 F]aluminum fluoride coordination reaction, which was first developed on the flexible GE TRACERLab FX2N (FXN) platform and later translated to the cassette-based module Trasis AllInOne (AIO). The results obtained with both modules were comparable in terms of yield and reproducibility. Automation of [18 F]FAPI-74 radiosynthesis on the FXN was carried out in 35 min with a radiochemical yield (RCY) of 18.5 ± 2.5% (n = 5, relative to starting [18 F]fluoride). Method transfer to the AIO platform following minor optimizations allowed for the production of [18 F]FAPI-74 in an isolated RCY of 20 ± 2.5% [n = 3] with an overall synthesis time of 40 min. The radiochemical purity was greater than 95% for [18 F]FAPI-74, obtained from both modules. Overall, the protocol reliably provides a sterile and pyrogen-free good manufacturing practice (GMP) compliant product of [18 F]FAPI-74 suitable for clinical PET imaging. |
| تدمد: | 1099-1344 0362-4803 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::ed2c38e30a13ff4d49842955a3fa2055Test https://doi.org/10.1002/jlcr.3926Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........ed2c38e30a13ff4d49842955a3fa2055 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10991344 03624803 |
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