التفاصيل البيبلوغرافية
| العنوان: |
miR-635 targets KIFC1 to inhibit the progression of gastric cancer. |
| المؤلفون: |
Feng-Yu Cao, Yong-Bin Zheng, Chao Yang, Su-Yang Huang, Xiao-Bo He, Shi-Lun Tong, Cao, Feng-Yu, Zheng, Yong-Bin, Yang, Chao, Huang, Su-Yang, He, Xiao-Bo, Tong, Shi-Lun |
| المصدر: |
Journal of Investigative Medicine (Sage Publications Inc.); Dec2020, Vol. 68 Issue 8, p1357-1363, 7p |
| مستخلص: |
Accumulating studies have shown that the dysregulation of microRNAs is related to the carcinogenesis and development of gastric cancer (GC), and the role of miR-635 in GC remains largely unknown. miR-635 and Kinesin Family Member C1 (KIFC1) mRNA expression in GC tissues and paracancerous tissues and cells were detected by quantitative real-time PCR. KIFC1 protein expression in GC tissues and paracancerous normal tissues and cells was detected by immunohistochemistry and western blot. Cell proliferation was monitored by Cell Counting Kit-8 assay and 5-bromo-2'-deoxyuridine assay. Transwell assay was employed to detect the migration and invasion of GC cells. The dual-luciferase reporter gene assay was adopted to detect the targeting relationship between miR-635 and KIFC1. Compared with paracancerous tissues, miR-635 expression was remarkably decreased in GC tissues; conversely, KIFC1 expression was significantly increased. Compared with human normal gastric epithelial cell GSE-1, miR-635 expression was markedly decreased in GC cell lines. Meanwhile, KIFC1 expression was significantly increased, and the Kaplan-Meier Plotter database showed that its high expression was remarkably associated with poor prognosis. Additionally, miR-635 can negatively regulate KIFC1. miR-635 can target KIFC1 to inhibit proliferation, migration and invasion of GC cells. Collectively, miR-635 is lowly expressed in GC, and it inhibits proliferation, migration and invasion of GC cells via regulating KIFC1. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
