Cecropins as a marker of Spodoptera frugiperda immunosuppression during entomopathogenic bacterial challenge
| العنوان: | Cecropins as a marker of Spodoptera frugiperda immunosuppression during entomopathogenic bacterial challenge |
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| المؤلفون: | Jean-Michel Escoubas, Alain Givaudan, Z. Abi Khattar, Sylvie Pages, Bernard Duvic, N.-A. Volkoff, Véronique Jouan, Delphine Destoumieux-Garzón, N. Essa, Pierre-Alain Girard |
| المساهمون: | Diversité, Génomes et Interactions Microorganismes-Insectes, Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Université Montpellier 2 - Sciences et Techniques (UM2), Ecologie des systèmes marins côtiers (Ecosym), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 1 (UM1), French Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique |
| المصدر: | Journal Of Insect Physiology (0022-1910) (Pergamon-elsevier Science Ltd), 2012-06, Vol. 58, N. 6, P. 881-888 Journal of Insect Physiology Journal of Insect Physiology, Elsevier, 2012, 58 (6), pp.881-888. ⟨10.1016/j.jinsphys.2012.04.001⟩ |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | 0106 biological sciences, Physiology, [SDV]Life Sciences [q-bio], Xenorhabdus, medicine.disease_cause, 01 natural sciences, Hemolymph, INFECTION, Invertebrate, Phylogeny, Xenorhabdus mauleonii, Innate immunity, 0303 health sciences, biology, Cecropins, MANDUCA-SEXTA, Antimicrobial, FAMILY, DROSOPHILA, SILKWORM BOMBYX-MORI, ANTIBACTERIAL PEPTIDES, Lepidopteran, EXPRESSION, Molecular Sequence Data, Microbial Sensitivity Tests, Spodoptera, Microbiology, 03 medical and health sciences, medicine, Animals, Amino Acid Sequence, 030304 developmental biology, Molecular mass, Base Sequence, XENORHABDUS-NEMATOPHILA, fungi, Sequence Analysis, DNA, biology.organism_classification, ANTIMICROBIAL PEPTIDES, 010602 entomology, Humoral immunity, Cecropin, Insect Science, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, INSECT IMMUNITY, Sequence Alignment, Bacteria, Immunosuppression |
| الوصف: | International audience; An antimicrobial peptide (AMP) of the cecropin family was isolated by HPLC from plasma of the insect pest, Spodoptera frugiperda. Its molecular mass is 3910.9 Da as determined by mass spectrometry. Thanks to the EST database Spodobase, we were able to describe 13 cDNAs encoding six different cecropins which belong to the sub-families CecA, CecB, CecC and CecD. The purified peptide identified as CecB1 was chemically synthesized (syCecB1). It was shown to be active against Gram-positive and Gram-negative bacteria as well as fungi. Two closely related entomopathogenic bacteria, Xenorhabdus nematophila F1 and Xenorhabdus mauleonii VC01(T) showed different susceptibility to syCecB1. Indeed, X. nematophila was sensitive to syCecB1 whereas X. mauleonii had a minimal inhibitory concentration (MIC) eight times higher. Interestingly, injection of live X. nematophila into insects did not induce the expression of AMPs in hemolymph. This effect was not observed when this bacterium was heat-killed before injection. On the opposite, both live and heat-killed X. mauleonii induced the expression of AMPs in the hemolymph of S. frugiperda. The same phenomenon was observed for another immune-related protein lacking antimicrobial activity. Altogether, our data suggest that Xenorhabdus strains have developed different strategies to supplant the humoral defense mechanisms of S. frugiperda, either by increasing their resistance to AMPs or by preventing their expression during such host-pathogen interaction. (C) 2012 Elsevier Ltd. All rights reserved. |
| وصف الملف: | application/pdf |
| تدمد: | 1879-1611 0022-1910 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::acf8713c128e297891dcf295b9eb9b41Test https://pubmed.ncbi.nlm.nih.gov/22487443Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....acf8713c128e297891dcf295b9eb9b41 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18791611 00221910 |
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