التفاصيل البيبلوغرافية
| العنوان: |
Ultrasonic-enhanced gentamicin transport through colony biofilms of Pseudomonas aeruginosaand Escherichia coli |
| المؤلفون: |
Carmen, John C., Runyan, Christopher M., Robison, Rachel A., Nelson, Jared L., Beckstead, Benjamin L., Pitt, William G., Schaalje, G. Bruce |
| المصدر: |
Journal of Infection and Chemotherapy; January 2004, Vol. 10 Issue: 4 p193-199, 7p |
| مستخلص: |
The hypothesis that ultrasound increases antibiotic transport through biofilms of Escherichia coliand Pseudomonas aeruginosawas investigated using colony biofilms. Biofilms grown on membrane filters were transferred to nutrient agar containing 50μg/ml gentamicin. A smaller filter was placed on top of the biofilm and a blank concentration disk was situated atop the filter. Diffusion of antibiotic through the biofilms was allowed for 15, 30, or 45min at 37°C. Some of these biofilms were exposed to 70-kHz ultrasound and others were not. Each concentration disk was then placed on a nutrient agar plate spread with a lawn of E. coli. The resulting zone of inhibition was used to calculate the amount of gentamicin that was transported through the biofilm into the disk. The E. coliand P. aeruginosabiofilms grown for 13 and 24h were exposed to two different ultrasonic power densities. Ultrasonication significantly increased the transport of gentamicin through the biofilm. Insonation of biofilms of E. colifor 45min more than doubled the amount of gentamicin compared to their noninsonated counterparts. For P. aeruginosabiofilms, no detectable gentamicin penetrated the biofilm within 45min without ultrasound; however, when insonated (1.5W/cm2) for 45min, the disks collected more than 0.45μg antibiotic. Ultrasonication significantly increased transport of gentamicin across biofilms that normally blocked or slowed gentamicin transport when not exposed to ultrasound. This enhanced transport may be partially responsible for the increased killing of biofilm bacteria exposed to combinations of antibiotic and ultrasound. |
| قاعدة البيانات: |
Supplemental Index |
