A gene located downstream of the clavulanic acid gene cluster in Streptomyces clavuligerus ATCC 27064 encodes a putative response regulator that affects clavulanic acid production
| العنوان: | A gene located downstream of the clavulanic acid gene cluster in Streptomyces clavuligerus ATCC 27064 encodes a putative response regulator that affects clavulanic acid production |
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| المؤلفون: | Susan E. Jensen, Kye Joon Lee, Dae Wi Kim, Ju Yeon Song, Eun Sook Kim |
| المصدر: | Journal of Industrial Microbiology & Biotechnology. 36:301-311 |
| بيانات النشر: | Oxford University Press (OUP), 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Mutant, Streptomyces clavuligerus, Bioengineering, Applied Microbiology and Biotechnology, Open Reading Frames, Bacterial Proteins, Sigma factor, Clavulanic acid, Gene cluster, medicine, Cephamycins, Clavulanic Acid, biology, Gene Expression Regulation, Bacterial, biology.organism_classification, Streptomyces, Anti-Bacterial Agents, Culture Media, Open reading frame, Response regulator, Biochemistry, Multigene Family, Mutation, Cephamycin, Biotechnology, medicine.drug |
| الوصف: | Three open reading frames denoted as orf21, orf22, and orf23 were identified from downstream of the currently recognized gene cluster for clavulanic acid biosynthesis in Streptomyces clavuligerus ATCC 27064. The new orfs were annotated after in silico analysis as genes encoding a putative sigma factor, a sensor kinase, and a response regulator. The roles of the individual genes were explored by disruption of the corresponding orfs, and the morphological and antibiotic production phenotypes of the resulting mutants were compared. In orf21 and orf22 mutants, no growth or morphological differences were noted, but modest reduction of cephamycin C (orf21), or both cephamycin C and clavulanic acid production (orf22) compared with wild-type, were observed. In orf23 mutant, cell growth and sporulation was retarded, and clavulanic acid and cephamycin C production were reduced to 40 and 47% of wild-type levels, respectively. Conversely, overexpression of orf23 caused precocious hyperproduction of spores on solid medium, and antibiotic production was increased above the levels seen in plasmid control cultures. Transcriptional analyses were also carried out on orf23 and showed that mutation had little effect on transcription of genes associated with the early stages of cephamycin C or clavulanic acid production but transcription of claR, which regulates the late stages of clavulanic acid production, was reduced in orf23 mutants. These observations suggest that the orf23 product may enable S. clavuligerus to respond to environmental changes by altering cell growth and differentiation. In addition, the effects of ORF23 on growth might indirectly regulate the biosynthesis of secondary metabolites such as clavulanic acid and cephamycin C. |
| تدمد: | 1476-5535 1367-5435 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1024de32fd862fbb11697f379982bc7Test https://doi.org/10.1007/s10295-008-0499-2Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b1024de32fd862fbb11697f379982bc7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765535 13675435 |
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