التفاصيل البيبلوغرافية
| العنوان: |
Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method. |
| المؤلفون: |
Zeman, David1,2 (AUTHOR) david.zeman@fno.cz, Kušnierová, Pavlína3 (AUTHOR), Bojková, Jana4 (AUTHOR), Všianský, František3 (AUTHOR), Zapletalová, Olga2 (AUTHOR) |
| المصدر: |
Journal of Immunoassay & Immunochemistry. 2017, Vol. 38 Issue 2, p165-177. 13p. |
| مصطلحات موضوعية: |
*IMMUNOGLOBULIN G, *CEREBROSPINAL fluid, *ENZYME-linked immunosorbent assay, *IMMUNOGLOBULINS, *IMMUNOBLOTTING |
| مستخلص: |
IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using Hevylite™ antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was observed (increased in 16 and decreased in 3 cases). The analysis of light chain composition of intrathecally synthesised immunoglobulins could contribute to our understanding of intrathecal humoral immune response, although its diagnostic utility is limited. [ABSTRACT FROM PUBLISHER] |
| قاعدة البيانات: |
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