Unexpectedly low nitrogen acquisition and absence of root architecture adaptation to nitrate supply in a Medicago truncatula highly branched root mutant
| العنوان: | Unexpectedly low nitrogen acquisition and absence of root architecture adaptation to nitrate supply in a Medicago truncatula highly branched root mutant |
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| المؤلفون: | Marc Lepetit, Françoise Jacquin, Marie-Laure Martin-Magniette, Sandrine Balzergue, Grégoire Aubert, Nathalie G. Munier-Jolain, Christophe Salon, Geoffroy Lescure, Henri de Larambergue, Sylvie Citerne, Virginie Bourion, Chantal Martin, Gérard Duc |
| المساهمون: | Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Mathématiques et Informatique Appliquées (MIA-Paris), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Institut Jean-Pierre Bourgin (IJPB), Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), INRA (AgroBI programme), Burgundy Council (PARI-Agrale6 programme), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Unité de recherche en génomique végétale ( URGV ), Institut National de la Recherche Agronomique ( INRA ) -Université d'Évry-Val-d'Essonne ( UEVE ) -Centre National de la Recherche Scientifique ( CNRS ), Mathématiques et Informatique Appliquées ( MIA-Paris ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, ERL8196 UMR URGV, Centre National de la Recherche Scientifique ( CNRS ), Institut Jean-Pierre Bourgin ( IJPB ), Laboratoire des symbioses tropicales et méditerranéennes ( LSTM ), Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( CIRAD ) -Université Montpellier 1 ( UM1 ) -Institut National de la Recherche Agronomique ( INRA ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Université de Montpellier ( UM ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Bourion, Virginie |
| المصدر: | Journal of Experimental Botany Journal of Experimental Botany, 2014, 65 (9), pp.2365-2380. ⟨10.1093/jxb/eru124⟩ Journal of Experimental Botany, Oxford University Press (OUP), 2014, 65 (9), pp.2365-2380. ⟨10.1093/jxb/eru124⟩ Journal of Experimental Botany, Oxford University Press (OUP), 2014, 65 (9), pp.2365-2380. 〈10.1093/jxb/eru124〉 Journal of Experimental Botany 9 (65), 2365-2380. (2014) |
| بيانات النشر: | Oxford University Press (OUP), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Nitrogen, Physiology, [SDV]Life Sciences [q-bio], Mutant, phenylpropanoid, Plant Science, root architecture, phénylpropanoïde, Plant Roots, Transcriptome, chemistry.chemical_compound, Nitrate, Symbiosis, architecture racinaire, Medicago truncatula, Botany, nitrogen acquisition, Gene, Plant Proteins, 2. Zero hunger, Nitrates, [ SDV ] Life Sciences [q-bio], biology, Phenylpropanoid, nitrogen limitation, Wild type, biology.organism_classification, acide aminé, acquisition d'azote, chemistry, Amino acids, highly branched root mutant, Research Paper |
| الوصف: | Summary Physiological and developmental analyses provide evidence that the highly branched root architecture of a mutant results from systemic regulation by its nitrogen status, possibly involving glutamine or asparagine signals. To complement N2 fixation through symbiosis, legumes can efficiently acquire soil mineral N through adapted root architecture. However, root architecture adaptation to mineral N availability has been little studied in legumes. Therefore, this study investigated the effect of nitrate availability on root architecture in Medicago truncatula and assessed the N-uptake potential of a new highly branched root mutant, TR185. The effects of varying nitrate supply on both root architecture and N uptake were characterized in the mutant and in the wild type. Surprisingly, the root architecture of the mutant was not modified by variation in nitrate supply. Moreover, despite its highly branched root architecture, TR185 had a permanently N-starved phenotype. A transcriptome analysis was performed to identify genes differentially expressed between the two genotypes. This analysis revealed differential responses related to the nitrate acquisition pathway and confirmed that N starvation occurred in TR185. Changes in amino acid content and expression of genes involved in the phenylpropanoid pathway were associated with differences in root architecture between the mutant and the wild type. |
| وصف الملف: | application/pdf |
| تدمد: | 1460-2431 0022-0957 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc7fa88bc1372b18bfe17a352e2a11e8Test https://doi.org/10.1093/jxb/eru124Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bc7fa88bc1372b18bfe17a352e2a11e8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602431 00220957 |
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