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1دورية أكاديمية
المؤلفون: Waters, Brian M, Uauy, Cristobal, Dubcovsky, Jorge, Grusak, Michael A
المصدر: Journal of Experimental Botany. 60(15)
مصطلحات موضوعية: Genetics, Prevention of disease and conditions, and promotion of well-being, 3.3 Nutrition and chemoprevention, Cardiovascular, Stroke, Metabolic and endocrine, Cancer, Biological Transport, Gene Expression Regulation, Plant, Iron, Nitrogen, Plant Components, Aerial, Plant Proteins, Transcription Factors, Triticum, Zinc, Biofortification, grain protein content, iron, remobilization, senescence, zinc, Plant Biology, Crop and Pasture Production, Plant Biology & Botany
الوصف: The NAM-B1 gene is a NAC transcription factor that affects grain nutrient concentrations in wheat (Triticum aestivum). An RNAi line with reduced expression of NAM genes has lower grain protein, iron (Fe), and zinc (Zn) concentrations. To determine whether decreased remobilization, lower plant uptake, or decreased partitioning to grain are responsible for this phenotype, mineral dynamics were quantified in wheat tissues throughout grain development. Control and RNAi wheat were grown in potting mix and hydroponics. Mineral (Ca, Cu, Fe, K, Mg, Mn, P, S, and Zn) and nitrogen (N) contents of organs were determined at regular intervals to quantify the net remobilization from vegetative tissues and the accumulation of nutrients in grain. Total nutrient accumulation was similar between lines, but grain Fe, Zn, and N were at lower concentrations in the NAM knockdown line. In potting mix, net remobilization of N, Fe, and Zn from vegetative tissues was impaired in the RNAi line. In hydroponics with ample nutrients, net remobilization was not observed, but grain Fe and Zn contents and concentrations remained lower in the RNAi line. When Fe or Zn was withheld post-anthesis, both lines demonstrated remobilization. These results suggest that a major effect of the NAM genes is an increased efflux of nutrients from the vegetative tissues and a higher partitioning of nutrients to grain.
وصف الملف: application/pdf
الوصول الحر: https://escholarship.org/uc/item/7df2q1x5Test
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المساهمون: Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Approches intégratives du Transport Ionique (MINION), Département Biologie Cellulaire (BioCell), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, Département Biochimie, Biophysique et Biologie Structurale (B3S), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE20-0019,ISISTOR,Amélioration du contenu en fer de la graine(2016)
المصدر: Journal of Experimental Botany
Journal of Experimental Botany, 2019, 70 (3), pp.859--869. ⟨10.1093/jxb/ery388⟩
Journal of Experimental Botany, Oxford University Press (OUP), 2019, 70 (3), pp.859--869. ⟨10.1093/jxb/ery388⟩مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, metal, leaf senescence, Physiology, Iron, [SDV]Life Sciences [q-bio], education, Population, Mutant, Arabidopsis, Chromosomal translocation, Plant Science, 01 natural sciences, iron loading, 03 medical and health sciences, Nutrient, iron recycling, Autophagy, micronutrient, Arabidopsis thaliana, Micronutrients, health care economics and organizations, 57Fe, 2. Zero hunger, Manganese, education.field_of_study, biology, Chemistry, food and beverages, Biological Transport, 15. Life on land, Micronutrient, biology.organism_classification, Research Papers, Cell biology, Zinc, remobilization, premature senescence, 030104 developmental biology, Seeds, Growth and Development, fe-57, 010606 plant biology & botany
الوصف: Autophagy is an essential recycling mechanism making micronutrients available in vegetative organs for subsequent reallocation to seeds
Micronutrient deficiencies affect a large part of the world’s population. These deficiencies are mostly due to the consumption of grains with insufficient content of iron (Fe) or zinc (Zn). Both de novo uptake by roots and recycling from leaves may provide seeds with nutrients. Autophagy, which is a conserved mechanism for nutrient recycling in eukaryotes, was shown to be involved in nitrogen remobilization to seeds. Here, we have investigated the role of this mechanism in micronutrient translocation to seeds. We found that Arabidopsis thaliana plants impaired in autophagy display defects in nutrient remobilization to seeds. In the atg5-1 mutant, which is completely defective in autophagy, the efficiency of Fe translocation from vegetative organs to seeds was severely decreased even when Fe was provided during seed formation. Combining atg5-1 with the sid2 mutation that counteracts premature senescence associated with autophagy deficiency and using 57Fe pulse labeling, we propose a two-step mechanism in which Fe taken up de novo during seed formation is first accumulated in vegetative organs and subsequently remobilized to seeds. Finally, we show that translocation of Zn and manganese (Mn) to seeds is also dependent on autophagy. Fine-tuning autophagy during seed formation opens up new possibilities to improve micronutrient remobilization to seeds.الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03013692a7ae22f940869c2f5969c050Test
https://doi.org/10.1093/jxb/ery388Test -
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المؤلفون: Cristobal Uauy, Michael A. Grusak, Jorge Dubcovsky, Brian M. Waters
المصدر: Journal of experimental botany, vol 60, iss 15
Waters, BM; Uauy, C; Dubcovsky, J; & Grusak, MA. (2009). Wheat (Triticum aestivum) NAM proteins regulate the translocation of iron, zinc, and nitrogen compounds from vegetative tissues to grain. Journal of Experimental Botany, 60(15), 4263-4274. doi: 10.1093/jxb/erp257. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/7df2q1x5Testمصطلحات موضوعية: Crop and Pasture Production, grain protein content, senescence, Physiology, Nitrogen, Iron, Plant Biology & Botany, Biofortification, chemistry.chemical_element, Plant Biology, Plant Science, Zinc, Biology, Nutrient, Gene Expression Regulation, Plant, Genetics, Poaceae, Nitrogen cycle, Triticum, Plant Proteins, Phosphorus, zinc, food and beverages, Aerial, Biological Transport, Plant, Plant Components, Aerial, Hydroponics, Potting soil, Horticulture, remobilization, Agronomy, chemistry, Gene Expression Regulation, Plant Components, Transcription Factors
الوصف: The NAM-B1 gene is a NAC transcription factor that affects grain nutrient concentrations in wheat (Triticum aestivum). An RNAi line with reduced expression of NAM genes has lower grain protein, iron (Fe), and zinc (Zn) concentrations. To determine whether decreased remobilization, lower plant uptake, or decreased partitioning to grain are responsible for this phenotype, mineral dynamics were quantified in wheat tissues throughout grain development. Control and RNAi wheat were grown in potting mix and hydroponics. Mineral (Ca, Cu, Fe, K, Mg, Mn, P, S, and Zn) and nitrogen (N) contents of organs were determined at regular intervals to quantify the net remobilization from vegetative tissues and the accumulation of nutrients in grain. Total nutrient accumulation was similar between lines, but grain Fe, Zn, and N were at lower concentrations in the NAM knockdown line. In potting mix, net remobilization of N, Fe, and Zn from vegetative tissues was impaired in the RNAi line. In hydroponics with ample nutrients, net remobilization was not observed, but grain Fe and Zn contents and concentrations remained lower in the RNAi line. When Fe or Zn was withheld post-anthesis, both lines demonstrated remobilization. These results suggest that a major effect of the NAM genes is an increased efflux of nutrients from the vegetative tissues and a higher partitioning of nutrients to grain.
وصف الملف: application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57a096e7da438d870d6af3ddfafc1ed2Test
https://pubmed.ncbi.nlm.nih.gov/19858116Test
