miRNA-375 a Sensor of Glucotoxicity Is Altered in the Serum of Children with Newly Diagnosed Type 1 Diabetes
| العنوان: | miRNA-375 a Sensor of Glucotoxicity Is Altered in the Serum of Children with Newly Diagnosed Type 1 Diabetes |
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| المؤلفون: | Anne-Marie Madec, Sophie Rome, Lucien Marchand, Nicole Fabien, C. Thivolet, Audrey Jalabert, Kathleen Van den Hende, Emmanuelle Meugnier, Marc Nicolino |
| المساهمون: | Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Immunité infection vaccination (I2V), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-IFR128-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'endocrinologie pédiatrique [CHU Lyon-Hospices Civils de Lyon], Département Endocrinology et Diabétologie, Juvenile Diabetes Research Foundation (JDRF), NIH, Association for Diabetes Research (ARD), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Hôpital Femme Mère Enfant [CHU - HCL] (HFME), Service d'Endocrinologie Diabete Nutrition, Université de Lyon, Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Pillet, Lauriane, Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | Journal of diabetes research Journal of diabetes research, Hindawi Publishing Coporation, 2016, 2016, pp.1-7. ⟨10.1155/2016/1869082⟩ Journal of diabetes research, Hindawi Publishing Coporation, 2016, 2016, pp.1-7 Journal of Diabetes Research Journal of Diabetes Research, Vol 2016 (2016) Journal Of Diabetes Research, 1-8. (2016) Journal of diabetes research, 2016, 2016, pp.1-7. ⟨10.1155/2016/1869082⟩ |
| بيانات النشر: | Hindawi Limited, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, endocrine system diseases, [SDV]Life Sciences [q-bio], Endocrinology, Diabetes and Metabolism, lcsh:Diseases of the endocrine glands. Clinical endocrinology, Endocrinology, Child, ComputingMilieux_MISCELLANEOUS, geography.geographical_feature_category, nod mice, Islet, [SDV] Life Sciences [q-bio], medicine.anatomical_structure, beta-cell mass, micrornas, biomarkers, mir-375, autoimmune, mechanism, responses, strategy, disease, Biomarker (medicine), Female, Beta cell, Pancreas, Research Article, endocrine system, medicine.medical_specialty, Article Subject, Adolescent, Down-Regulation, Biology, Islets of Langerhans, 03 medical and health sciences, Downregulation and upregulation, Diabetes mellitus, Internal medicine, medicine, Humans, geography, Type 1 diabetes, lcsh:RC648-665, medicine.disease, Diabetes Mellitus, Type 1, Glucose, 030104 developmental biology, Immunology, Homeostasis |
| الوصف: | Background.The use of miRNAs as biomarkers for Type 1 Diabetes (T1D) risk is attractive as T1D is usually diagnosed in front of acute symptoms. As miR-375 is highly expressed in the endocrine pancreas, we postulated that its circulating level might reflect beta cell alterations and might be altered in the blood of T1D patients recently diagnosed.Methods.Sera were obtained from 22 T1D children at onset of the disease, before subcutaneous insulin treatment, and from 10 nondiabetic pediatric controls. MiR-375 seric level was quantified by stem-loop RT-PCR-based assay. MiRNAs regulations in isolated human islets in response to high glucose concentrations were determined by TaqMan Low-Density Array.Results.The abundance of miR-375, among the 410 miRNAs detected in human islets, mirrored its well-established role in rodent islet biology. Upregulated miRNAs targeted genes involved in islet homeostasis and regulation of beta cell mass. Downregulated miRNAs, including miR-375, were involved in pancreas secretion and protein turnover. Seric level of miR-375 was lower in T1D children versus age-matched controls, without any correlations with HbA1c, glycaemia, and number of autoantibodies.Conclusion.Altered circulating level of miR-375 at onset of T1D might be a general biomarker of metabolic alterations and inflammation associated with the disease. |
| وصف الملف: | text/xhtml; application/pdf |
| تدمد: | 2314-6753 2314-6745 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3989a33441cff8f908ec5d7af694cf0dTest https://doi.org/10.1155/2016/1869082Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3989a33441cff8f908ec5d7af694cf0d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146753 23146745 |
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