مورد إلكتروني
The relationships among bovine αS-casein phosphorylation isoforms suggest different phosphorylation pathways
| العنوان: | The relationships among bovine αS-casein phosphorylation isoforms suggest different phosphorylation pathways |
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| المصدر: | ISSN: 0022-0302 |
| بيانات النشر: | 2016 |
| تفاصيل مُضافة: | Fang, Z.H. Visker, M.H.P.W. Miranda, G. Delacroix-Buchet, A. Bovenhuis, H. Martin, P. |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Casein (CN) phosphorylation is an important posttranslational modification and is one of the key factors responsible for constructing and stabilizing casein micelles. Variation in phosphorylation degree of αS-CN is of great interest because it is suggested to affect milk technological properties. This study aimed to investigate the variation in phosphorylation degree of αS-CN among milk of individual cows and to explore relationships among different phosphorylation isoforms of αS-CN. For this purpose, we analyzed morning milk samples from 529 French Montbéliarde cows using liquid chromatography coupled with electrospray ionization mass spectrometry. We detected 3 new phosphorylation isoforms: αS2-CN-9P, αS2-CN-14P, and αS2-CN-15P in bovine milk, in addition to the known isoforms αS1-CN-8P, αS1-CN-9P, αS2-CN-10P, αS2-CN-11P, αS2-CN-12P, and αS2-CN-13P. The relative concentrations of each αS-CN phosphorylation isoform varied considerably among individual cows. Furthermore, the phenotypic correlations and hierarchical clustering suggest at least 2 regulatory systems for phosphorylation of αS-CN: one responsible for isoforms with lower levels of phosphorylation (αS1-CN-8P, αS2-CN-10P, and αS2-CN-11P), and another responsible for isoforms with higher levels of phosphorylation (αS1-CN-9P, αS2-CN-12P, αS2-CN-13P, and αS2-CN-14P). Identifying all phosphorylation sites of αS2-CN and investigating the genetic background of different αS2-CN phosphorylation isoforms may provide further insight into the phosphorylation mechanism of caseins. |
| مصطلحات الفهرس: | Casein, Liquid chromatography coupled with electrospray ionization mass spectrometry, Milk protein composition, Phosphorylation, Article/Letter to editor |
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| الإتاحة: | Open access content. Open access content Wageningen University & Research |
| ملاحظة: | application/pdf Journal of Dairy Science 99 (2016) 10 ISSN: 0022-0302 ISSN: 0022-0302 English |
| أرقام أخرى: | NLWUP oai:library.wur.nl:wurpubs/507128 https://library.wur.nl/WebQuery/wurpubs/507128Test 10.3168/jds.2016-11250 1200328323 |
| المصدر المساهم: | WUR STAFF PUBNS From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1200328323 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |