التفاصيل البيبلوغرافية
| العنوان: |
Dipyridamole enhances osteogenesis of three-dimensionally printed bioactive ceramic scaffolds in calvarial defects. |
| المؤلفون: |
Bekisz, Jonathan M., Flores, Roberto L., Witek, Lukasz, Lopez, Christopher D., Runyan, Christopher M., Torroni, Andrea, Cronstein, Bruce N., Coelho, Paulo G. |
| المصدر: |
Journal of Cranio-Maxillofacial Surgery; Feb2018, Vol. 46 Issue 2, p237-244, 8p |
| مصطلحات موضوعية: |
DIPYRIDAMOLE, BONE growth, BONE cells, TRAUMATIC bone defects, FIBRIN, PLATELET aggregation inhibitors, TISSUE scaffolds |
| مستخلص: |
Purpose The objective of this study was to test the osteogenic capacity of dipyridamole-loaded, three-dimensionally printed, bioactive ceramic (3DPBC) scaffolds using a translational, skeletally mature, large-animal calvarial defect model. Materials and methods Custom 3DPBC scaffolds designed to present lattice-based porosity only towards the dural surface were either coated with collagen (control) or coated with collagen and immersed in a 100 μM concentration dipyridamole (DIPY) solution. Sheep (n = 5) were subjected to 2 ipsilateral trephine-induced (11-mm diameter) calvarial defects. Either a control or a DIPY scaffold was placed in each defect, and the surgery was repeated on the contralateral side 3 weeks later. Following sacrifice, defects were evaluated through microcomputed tomography and histologic analysis for bone, scaffold, and soft tissue quantification throughout the defect. Parametric and non-parametric methods were used to determine statistical significance based on data distribution. Results No exuberant or ectopic bone formation was observed, and no histologic evidence of inflammation was noted within the defects. Osteogenesis was higher in DIPY-coated scaffolds compared to controls at 3 weeks (p = 0.013) and 6 weeks (p = 0.046) in vivo . When bone formation was evaluated as a function of defect radius, average bone formation was higher for DIPY relative to control scaffolds at both time points (significant at defect central regions at 3 weeks and at margins at 6 weeks, p = 0.046 and p = 0.031, respectively). Conclusion Dipyridamole significantly improves the calvarial bone regeneration capacity of 3DPBC scaffolds. The most significant difference in bone regeneration was observed centrally within the interface between the 3DPBC scaffold and the dura mater. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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