Effect of low-level laser therapy on the release of interleukin-6 and basic fibroblast growth factor from cultured human skin fibroblasts in normal and high glucose mediums
| العنوان: | Effect of low-level laser therapy on the release of interleukin-6 and basic fibroblast growth factor from cultured human skin fibroblasts in normal and high glucose mediums |
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| المؤلفون: | Mohammad Bayat, Mohammad Esmaeelinejad |
| المصدر: | Journal of Cosmetic and Laser Therapy. 15:310-317 |
| بيانات النشر: | Informa UK Limited, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | medicine.medical_specialty, medicine.medical_treatment, Basic fibroblast growth factor, Human skin, Dermatology, Cell Line, chemistry.chemical_compound, Laser therapy, Internal medicine, Humans, Medicine, Statistical analysis, Low-Level Light Therapy, Interleukin 6, Low level laser therapy, Skin, biology, business.industry, Interleukins, Fibroblasts, Culture Media, Glucose, Endocrinology, chemistry, High glucose, biology.protein, Fibroblast Growth Factor 2, Surgery, Once daily, business |
| الوصف: | This study evaluated the effects of low-level laser therapy (LLLT) on human skin fibroblasts (HSFs) that have been cultured in high glucose concentration media.HSFs were cultured under physiological glucose condition medium, and then cultured in high glucose concentration medium (15 mM/L) for 1 or 2 weeks prior to LLLT. Experimental HSFs were irradiated with three energy densities (0.5, 1, and 2 J/cm(2)) once daily for three consecutive days. Release of interleukin-6 (IL-6) and basic fibroblast growth factor (bFGF) was evaluated using the enzyme-linked immunosorbent assay (ELISA) method.Statistical analysis showed three doses of 0.5 (p = 0.049), 1 (p = 0.027), and 2 J/cm(2) (p = 0.004) stimulated the release of IL-6 in HSFs cultured in high glucose concentration medium compared with that of non-irradiated HSFs that were cultured in the same medium. LLLT with 2 J/cm(2) induced the release of bFGF from HSFs cultured in high glucose concentration medium for 1 or 2 weeks (both p = 0.04).Our study showed that LLLT stimulated the release of IL-6 and bFGF from HSFs cultured in high glucose concentration medium. LLLT was more effective in releasing IL-6 and bFGF while HSFs which were cultured in physiologic glucose concentration medium during laser irradiation. |
| تدمد: | 1476-4180 1476-4172 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92b92435420cc745301a7745a70c0ca7Test https://doi.org/10.3109/14764172.2013.803366Test |
| رقم الانضمام: | edsair.doi.dedup.....92b92435420cc745301a7745a70c0ca7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14764180 14764172 |
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