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المؤلفون: Cynthia M. Grimsley-Myers, Noelle D. Dwyer, Ayushma Shrestha, David R. Beier, Carlita B. Favero, Rasha N. Henshaw
المصدر: Journal of Comparative Neurology. 521:677-696
مصطلحات موضوعية: Male, Mutant, Gestational Age, Biology, Article, Mice, Mice, Neurologic Mutants, Prosencephalon, Thalamus, Pregnancy, Neural Pathways, medicine, Animals, Genetic Testing, Axon, Endoplasmic Reticulum Chaperone BiP, Cells, Cultured, Heat-Shock Proteins, Cerebral Cortex, Mammals, Cerebrum, General Neuroscience, Endoplasmic reticulum, Axon extension, Fibroblasts, Axons, Mice, Inbred C57BL, medicine.anatomical_structure, nervous system, Forebrain, Female, Axon guidance, Neuroscience, Genetic screen
الوصف: Proper development of axonal connections is essential for brain function. A forward genetic screen for mice with defects in thalamocortical development previously isolated a mutant called baffled. Here we describe the axonal defects of baffled in further detail and identify a point mutation in the Hspa5 gene, encoding the endoplasmic reticulum chaperone BiP/GRP78. This hypomorphic mutation of BiP disrupts proper development of the thalamocortical axon projection and other forebrain axon tracts, as well as cortical lamination. In baffled mutant brains, a reduced number of thalamic axons innervate the cortex by the time of birth. Thalamocortical and corticothalamic axons are delayed, overfasciculated, and disorganized along their pathway through the ventral telencephalon. Furthermore, dissociated mutant neurons show reduced axon extension in vitro. Together, these findings demonstrate a sensitive requirement for the endoplasmic reticulum chaperone BiP/GRP78 during axon outgrowth and pathfinding in the developing mammalian brain. J. Comp. Neurol. 521:677–696, 2013. © 2012 Wiley Priodcals, Inc.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd7381424e4459fb237caf4a62caef53Test
https://doi.org/10.1002/cne.23199Test -
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المؤلفون: Enjin, Anders, Rabe, Nadine, 1978, Nakanishi, Stan, Vallstedt, Anna, 1974, Gezelius, Henrik, 1977, Memic, Fatima, Lind, Magnus, Hjalt, Tord, Tourtellotte, Warren, Bruder, Carl, Eichele, Gregor, Whelan, Patrick J, Kullander, Klas, 1966
المصدر: Journal of Comparative Neurology. 518(12):2284-2304
مصطلحات موضوعية: mouse genetics, neuronal network, interneuron, motor neuron, spinal cord, genetic screen, MEDICINE, MEDICIN, Medicinsk utvecklings- och neurobiologi, Developmental Neurosciences, Genetics, Genetik
الوصف: Spinal cholinergic neurons are critical for motor function in both the autonomic and somatic nervous systems and are affected in spinal cord injury and in diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy. Using two screening approaches and in situ hybridization, we identified 159 genes expressed in typical cholinergic patterns in the spinal cord. These include two general cholinergic neuron markers, one gene exclusively expressed in motor neurons and nine genes expressed in unknown subtypes of somatic motor neurons. Further, we present evidence that Chondrolectin (Chodl) is a novel genetic marker for putative fast motor neurons and that estrogen-related receptor b (ERRb) is a candidate genetic marker for slow motor neurons. In addition, we suggest paired-like homeodomain transcription factor 2 (Pitx2) as a marker for cholinergic partition cells.
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