Quantitative Methylation-Specific Polymerase Chain Reaction Gene Patterns in Urine Sediment Distinguish Prostate Cancer Patients From Control Subjects
| العنوان: | Quantitative Methylation-Specific Polymerase Chain Reaction Gene Patterns in Urine Sediment Distinguish Prostate Cancer Patients From Control Subjects |
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| المؤلفون: | William H. Westra, Shahnaz Begum, Wim Van Criekinge, Rui Henrique, David Sidransky, Eli Rosenbaum, Ozlem Topaloglu, Mohammad O. Hoque |
| المصدر: | Journal of Clinical Oncology. 23:6569-6575 |
| بيانات النشر: | American Society of Clinical Oncology (ASCO), 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Adult, Male, PCA3, Cancer Research, Pathology, medicine.medical_specialty, Urinalysis, Polymerase Chain Reaction, Sensitivity and Specificity, law.invention, GSTP1, Prostate cancer, Reference Values, Prostate, law, Biomarkers, Tumor, Humans, Medicine, Genes, Tumor Suppressor, Promoter Regions, Genetic, Polymerase chain reaction, Aged, Probability, Aged, 80 and over, medicine.diagnostic_test, business.industry, Prostatic Neoplasms, Promoter, DNA, Neoplasm, DNA Methylation, Middle Aged, medicine.disease, Neoplasm Proteins, Gene Expression Regulation, Neoplastic, Logistic Models, medicine.anatomical_structure, Oncology, Case-Control Studies, DNA methylation, Cancer research, business |
| الوصف: | Purpose Aberrant promoter hypermethylation of several known or putative tumor suppressor genes occurs frequently during the pathogenesis of prostate cancers and is a promising marker for cancer detection. We sought to develop a test for prostate cancer based on a quantitative methylation-specific polymerase chain reaction (QMSP) of multiple genes in urine sediment DNA. Patients and Methods We tested urine sediment DNA for aberrant methylation of nine gene promoters (p16INK4a, p14ARF, MGMT, GSTP1, RARβ2, CDH1 [E-cadherin], TIMP3, Rassf1A, and APC) from 52 patients with prostate cancer and 21 matched primary tumors by quantitative fluorogenic real-time polymerase chain reaction. We also analyzed urine sediments from 91 age-matched individuals without any history of genitourinary malignancy as controls. Results Promoter hypermethylation of at least one of the genes studied was detected in urine samples from all 52 prostate cancer patients. Urine samples from the 91 controls without evidence of genitourinary cancer revealed no methylation of the p16, ARF, MGMT, and GSTP1 gene promoters, whereas methylation of RARβ2, TIMP3, CDH1, Rassf1A, and APC was detected at low levels. Conclusion Overall, methylation found in urine samples matched the methylation status in the primary tumor. A combination of only four genes (p16, ARF, MGMT, and GSTP1) would theoretically allow us to detect 87% of prostate cancers with 100% specificity. Our data support further development of the noninvasive QMSP assay in urine DNA for early detection and surveillance of prostate cancer. |
| تدمد: | 1527-7755 0732-183X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5dd5570edc63dc60e65449fb753bad8aTest https://doi.org/10.1200/jco.2005.07.009Test |
| رقم الانضمام: | edsair.doi.dedup.....5dd5570edc63dc60e65449fb753bad8a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15277755 0732183X |
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