Regulation of Angiogenesis Discriminates Tissue Resident MSCs from Effective and Defective Osteogenic Environments †
| العنوان: | Regulation of Angiogenesis Discriminates Tissue Resident MSCs from Effective and Defective Osteogenic Environments † |
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| المؤلفون: | Elena Jones, Sarah M Churchman, Pinelopi Kastana, Dennis McGonagle, Clara Sanjurjo-Rodríguez, Richard Cuthbert, Payal Ganguly, Peter V. Giannoudis, Ahmed Lotfy, Evangelia Papadimitriou, Hiang Boon Tan |
| المصدر: | Journal of Clinical Medicine Journal of Clinical Medicine, Vol 9, Iss 1628, p 1628 (2020) RUC. Repositorio da Universidade da Coruña instname Volume 9 Issue 6 RUC: Repositorio da Universidade da Coruña Universidade da Coruña (UDC) |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Angiogenesis, Long bone, lcsh:Medicine, regenerative medicine, Fracture healing, MSCs, Bone healing, Regenerative medicine, Non-union, Article, osteogenesis, 03 medical and health sciences, 0302 clinical medicine, Osteogenesis, medicine, Induced periosteum, 030304 developmental biology, 0303 health sciences, Periosteum, business.industry, Mesenchymal stem cell, lcsh:R, General Medicine, fracture healing, Cell biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Bone marrow, induced periosteum, business, Blood vessel, non-union |
| الوصف: | [Abstract] Background: The biological mechanisms that contribute to atrophic long bone non-union are poorly understood. Multipotential mesenchymal stromal cells (MSCs) are key contributors to bone formation and are recognised as important mediators of blood vessel formation. This study examines the role of MSCs in tissue formation at the site of atrophic non-union. Materials and Methods: Tissue and MSCs from non-union sites (n = 20) and induced periosteal (IP) membrane formed following the Masquelet bone reconstruction technique (n = 15) or bone marrow (n = 8) were compared. MSC content, differentiation, and influence on angiogenesis were measured in vitro. Cell content and vasculature measurements were performed by flow cytometry and histology, and gene expression was measured by quantitative polymerase chain reaction (qPCR). Results: MSCs from non-union sites had comparable differentiation potential to bone marrow MSCs. Compared with induced periosteum, non-union tissue contained similar proportion of colony-forming cells, but a greater proportion of pericytes (p = 0.036), and endothelial cells (p = 0.016) and blood vessels were more numerous (p = 0.001) with smaller luminal diameter (p = 0.046). MSCs showed marked differences in angiogenic transcripts depending on the source, and those from induced periosteum, but not non-union tissue, inhibited early stages of in vitro angiogenesis. Conclusions: In vitro, non-union site derived MSCs have no impairment of differentiation capacity, but they differ from IP-derived MSCs in mediating angiogenesis. Local MSCs may thus be strongly implicated in the formation of the immature vascular network at the non-union site. Attention should be given to their angiogenic support profile when selecting MSCs for regenerative therapy. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2077-0383 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2391a39e99227eb939c3c8b44cb90e16Test http://europepmc.org/articles/PMC7355658Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2391a39e99227eb939c3c8b44cb90e16 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20770383 |
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