المؤلفون: Xuyang Gong, Ailong Huang

المصدر: Journal of Clinical Laboratory Analysis

مصطلحات موضوعية: 0301 basic medicine, Male, alpha‐fetoprotein, Lymphocyte, Clinical Biochemistry, Tropomyosin, Logistic regression, Gastroenterology, Cohort Studies, 0302 clinical medicine, Immunology and Allergy, Research Articles, biology, Liver Neoplasms, Hematology, hepatocellular carcinoma, Middle Aged, Medical Laboratory Technology, medicine.anatomical_structure, MutS Homolog 2 Protein, MutS homologs 2, Liver, 030220 oncology & carcinogenesis, Hepatocellular carcinoma, Cohort, tropomyosin‐4, Female, alpha-Fetoproteins, Alpha-fetoprotein, Research Article, Microbiology (medical), Adult, medicine.medical_specialty, Carcinoma, Hepatocellular, 03 medical and health sciences, Internal medicine, medicine, Humans, Neutrophil to lymphocyte ratio, Interleukin 6, Aged, P53, business.industry, Biochemistry (medical), Public Health, Environmental and Occupational Health, medicine.disease, digestive system diseases, 030104 developmental biology, biology.protein, Differential diagnosis, Tumor Suppressor Protein p53, business

الوصف: Background Current study aimed to explore the value of P53, MutS homologs 2 (MSH2), and tropomyosin‐4 (Tm‐4) combined with inflammatory factors, life‐history traits in the differential diagnosis of alpha‐fetoprotein‐negative hepatocellular carcinoma (AFP‐Negative HCC). Methods A testing cohort including 280 AFP‐Negative HCC patients and 300 controls was included. Three external validation cohorts from 3 centers were used to assess the novel logistic regression model including 400 AFP‐Negative HCC patients and 400 controls. Results Compared with the control group, the levels of P53, MSH2, and Tm‐4 protein in si‐P53 group, si‐MSH2 group, and si‐Tm‐4 group were significantly reduced (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f2a1e5ce0d7d561add5265c0d0f8d16Test
https://pubmed.ncbi.nlm.nih.gov/32363617Test

المؤلفون: Lili Zhou, Siyuan Chen, Tan Xiaodan, Lingxiu Ma, Qi Xu, Qin Hongyan, Mo Yuncong, Junhong Li, Zhixiao Wei

المصدر: Journal of Clinical Laboratory Analysis

مصطلحات موضوعية: Liver Cirrhosis, 0301 basic medicine, Microbiology (medical), medicine.medical_specialty, Carcinoma, Hepatocellular, alpha‐fetoprotein, Cirrhosis, Clinical Biochemistry, Sensitivity and Specificity, Gastroenterology, Hepatitis, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Biomarkers, Tumor, Humans, Immunology and Allergy, Medicine, AFP-L3, neoplasms, Research Articles, business.industry, Liver Neoplasms, digestive, oral, and skin physiology, Biochemistry (medical), Significant difference, Public Health, Environmental and Occupational Health, hepatocellular carcinoma, Hematology, Lens culinaris agglutinin, AFP‐L3, medicine.disease, digestive system diseases, Medical Laboratory Technology, 030104 developmental biology, meta‐analysis, Case-Control Studies, 030220 oncology & carcinogenesis, Hepatocellular carcinoma, Meta-analysis, Luminescent Measurements, embryonic structures, alpha-Fetoproteins, diagnostic value, Plant Lectins, business, Alpha-fetoprotein, Research Article

الوصف: Background To evaluate the clinical diagnostic efficacy of the combination of alpha‐fetoprotein (AFP) and lens culinaris agglutinin‐reactive fraction of AFP/total AFP (AFP‐L3%) for detecting hepatocellular carcinoma (HCC). Methods A comprehensive and systemic literature search was executed in Web of Science, PubMed, and the Cochrane Library websites. Then, the related articles were reviewed and the quality of included studies was evaluated with the QUADAS tool. Further, serum samples were collected from 49 HCC patients, 52 cirrhosis patients, 47 hepatitis patients, and 48 healthy controls and these samples were tested for AFP and AFP‐L3% levels. Results A total of 16 eligible articles were included in our meta‐analysis. The overall sensitivity (SEN) of AFP + AFP‐L3% was higher than that of AFP or AFP‐L3 alone; the overall specificity (SPE) of AFP + AFP‐L3% was lower than that of AFP or AFP‐L3 alone. In the original study, the related statistics were, respectively, SEN = 0.592 and SPE = 0.918 for AFP; SEN = 0.367 and SPE = 1.000 for AFP‐L3%; and SEN = 0.592 and SPE = 0.918 for the combination. Conclusion The results of meta‐analysis indicate there is a beneficial effect of using the unity of AFP and AFP‐L3% for HCC diagnosing. However, in the original study, just for the results of sensitivity and specificity, there is no significant difference between AFP alone and AFP + AFP‐L3%.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48edd2379086a8a0878fc7e306950fc0Test
https://doi.org/10.1002/jcla.23262Test

المؤلفون: Tanbo Yu, Zeyu Tian, Hongyan Wei, Ning Ning

المصدر: Journal of Clinical Laboratory Analysis

مصطلحات موضوعية: Male, 0301 basic medicine, alpha‐fetoprotein, Cirrhosis, diagnosis, Clinical Biochemistry, Logistic regression, Gastroenterology, 0302 clinical medicine, Immunology and Allergy, Research Articles, microRNA, Liver Neoplasms, hepatocellular carcinoma, Hep G2 Cells, Hematology, Middle Aged, Gene Expression Regulation, Neoplastic, Inorganic Pyrophosphatase, Medical Laboratory Technology, 030220 oncology & carcinogenesis, Hepatocellular carcinoma, Female, Prothrombin, alpha-Fetoproteins, Alpha-fetoprotein, Research Article, Adult, Microbiology (medical), medicine.medical_specialty, Carcinoma, Hepatocellular, protein induced by vitamin K deficiency or antagonist‐II, 03 medical and health sciences, Internal medicine, Vitamin K deficiency, medicine, Humans, Aged, Retrospective Studies, business.industry, Biochemistry (medical), Public Health, Environmental and Occupational Health, Reproducibility of Results, Cancer, Retrospective cohort study, medicine.disease, digestive system diseases, MicroRNAs, Logistic Models, 030104 developmental biology, Case-Control Studies, Vitamin K Deficiency, Differential diagnosis, business

الوصف: Background Alpha‐fetoprotein (AFP) has received extensive attention in the differential diagnosis of hepatocellular carcinoma (HCC), especially for AFP‐negative HCC (AFP‐NHCC). The current study aimed to explore the value of targeted regulation of LHPP expression‐related microRNAs (miRs) and protein induced by vitamin K deficiency or antagonist‐II (PIVKA‐II) in the differential diagnosis of AFP‐NHCC. Methods A retrospective study was conducted on a testing set—including 214 AFP‐NHCC patients, 200 cirrhosis, and 210 controls, and a validation set—including 140 AFP‐NHCC patients, 134 cirrhosis, and 128 controls recruited from The First Affiliated Hospital of Hunan Normal University. Serum miRs were examined using quantitative real‐time PCR method. Serum PIVKA‐II was measured by enzyme‐linked immunosorbent assay. Results Compared with adjacent tissues, LHPP protein levels in cancer tissues were significantly decreased (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ee8c731c42eced7a03fe9f35f047342Test
https://doi.org/10.1002/jcla.23071Test

المؤلفون: Qing Ye, Zhiyong Li, Weili Yin, Cuiping Xu, Fang Liu, Tao Han, Heping Zhao, Lina Wu, Ping Zhu, Ying Guo

المصدر: Journal of Clinical Laboratory Analysis

مصطلحات موضوعية: 0301 basic medicine, Male, Alcoholic liver disease, alpha‐fetoprotein, Clinical Biochemistry, Gastroenterology, 0302 clinical medicine, Immunology and Allergy, Age of Onset, liver stiffness measurement, Research Articles, Aged, 80 and over, education.field_of_study, Incidence (epidemiology), Liver Neoplasms, Hematology, Hepatitis C, hepatocellular carcinoma, Hepatitis B, Middle Aged, Medical Laboratory Technology, 030220 oncology & carcinogenesis, Hepatocellular carcinoma, Elasticity Imaging Techniques, Female, Liver cancer, Viral hepatitis, Research Article, alcoholic liver disease, Microbiology (medical), Adult, medicine.medical_specialty, Carcinoma, Hepatocellular, Population, viral hepatitis, 03 medical and health sciences, Young Adult, Age Distribution, Internal medicine, medicine, Humans, Sex Distribution, education, Liver Diseases, Alcoholic, Aged, business.industry, Biochemistry (medical), Public Health, Environmental and Occupational Health, medicine.disease, digestive system diseases, 030104 developmental biology, business

الوصف: Objective To determine the clinical and liver stiffness characteristics of a cohort of Chinese patients with Hepatocellular carcinoma in different stages of Barcelona clinic liver cancer. Methods Details of 1180 patients with Hepatocellular carcinoma referred from October 2014 to November 2017 were collected retrospectively. Demographic data, etiology, clinical, and biochemical details were retrospectively analyzed. The changes of liver stiffness in different etiologies and different stages of Barcelona clinic liver cancer were especially analyzed. Results The onset age was 60.33 ± 9.11 (range 24‐84) years, 9 cases were ≤40 years, 572 cases were 41‐60 years, males accounted for 83.92%, females accounted for 16.08%; 599 cases were ≥61 years, males accounted for 78.25%, females accounted for 21.75%. Compared with males, the proportion of females ≥61 is higher than that of men. Majority (n = 787; 66.69%) had HBV infection; second commonest cause was HCV infection (n = 217; 18.39%). More patients with HBV infection were 41‐60 years (69.06%) and were younger than HCV patients. There was no statistical difference in etiology, age, gender, and distribution of diabetes mellitus among different Barcelona clinic liver cancer stages (P > .05). The overall Hepatocellular carcinoma (HCC) was found to be positively correlated with alkaline phosphatase, γ‐glutamyltransferase, and alpha‐fetoprotein and liver stiffness measurement values from stage A to stage D (P 40 years old. Male is a high incidence population. In etiological analysis, HBV dominates HCC occurrence, HBV‐, HCV‐, and alcohol‐associated HCC have distinct clinical and biochemical characteristics, necessitating different screening policies to optimize HCC surveillance and management.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::890304edcd544189415d5d8a4d6f860cTest
https://pubmed.ncbi.nlm.nih.gov/31659795Test

المؤلفون: Rui-juan Ma, Shi-ni Qin, Weiwen Xu, Qiang Ma, Xi-Guang Xu, Cong-Rong Wang, Xiao-bo Wang, Ming Li, Juan-Ping Yu, Ming-Song Li

المصدر: Journal of Clinical Laboratory Analysis. 29:85-93

مصطلحات موضوعية: Microbiology (medical), medicine.medical_specialty, Pathology, Coefficient of variation, Clinical Biochemistry, Gastroenterology, Glypican 3, Antigen, Internal medicine, medicine, Immunology and Allergy, biology, business.industry, Biochemistry (medical), Public Health, Environmental and Occupational Health, Hematology, medicine.disease, digestive system diseases, Medical Laboratory Technology, Hepatocellular carcinoma, biology.protein, Biomarker (medicine), Antibody, Oncofetal antigen, business, Alpha-fetoprotein

الوصف: Background Glypican-3 (GPC3) is an oncofetal antigen that shows great promise as a biomarker for diagnosis of hepatocellular carcinoma (HCC), but there is no reliable kit that can be used to detect it in clinics. The aim of this study is to develop a stable performance kit for GPC3 detection in clinics. Design and methods The paired antibodies were identified through cycle-screening methods based on our previous research. Then, a double antibodies sandwich chemiluminescent immunoassay for detecting serum GPC3 was developed. The performance of the developed GPC3 diagnostic kit was evaluated by detecting the concentration of serum GPC3 and assessing its single or combined use with alpha fetoprotein (AFP) and cytokeratin 19 fragment (CK19) for HCC diagnosis. Results The assay demonstrated a linear range of 10–800 ng/ml, the cross-reactivity rate at 0.018% (AFP), 0.020% (carcino-embryonic antigen), and 0.021% (CK19), respectively. The minimum detectable concentration was 0.05 ng/ml; the intraassay coefficient of variation (CV) and interassay CV were both less than 10%, with good stability and reproducibility. GPC3 has a high sensitivity (54.2%) and specificity (99.4%) in diagnosing HCC. The level of GPC3 in HCC was robust higher than that in healthy or other liver diseases' sera (108.67 ± 230.04 ng/ml vs. 3.99 ± 7.68 ng/ml). The diagnostic sensitivity of GPC3 single or combined with CK19 and AFP for HCC was evaluated, and the rates were 54.2 and 90.6%, respectively. Conclusions An applicable chemiluminescent immunoassay with stable performance against GPC3 in diagnosing HCC has been established and the combination of GPC3 with CK19 and AFP could improve the diagnostic sensitivity for HCC.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::1e2b97fef7460fa19607526b0d127663Test
https://doi.org/10.1002/jcla.21733Test

المؤلفون: Jiang, Jingting, Wu, Changping, Xu, Ning, Yibei, Zhu, Jun, Wu, Mei, Ji, Bin, Xu, Nilsson-Ehle, Peter, Zhang, Xueguan

المصدر: Journal of Clinical Laboratory Analysis. 23(4):213-218

مصطلحات موضوعية: AFP-IgM immune complexes, hepatocellular carcinoma, tumor marker, alpha-fetoprotein, Medicin och hälsovetenskap, Medicinska och farmaceutiska grundvetenskaper, Farmakologi och toxikologi, Medical and Health Sciences, Basic Medicine, Pharmacology and Toxicology, Läkemedelskemi, Medicinal Chemistry

الوصف: We evaluated clinical significance of serum alpha-fetoprotein (AFP)-IgM immune complexes, in comparison with free AFP, on the diagnosis of primary hepatocellular carcinoma (HCC). Serum levels of AFP-IgM immune complexes and free AFP were determined by the ELISA method and electrochemiluminescence, respectively, in 103 healthy controls, 74 patients suffering from primary HCC, 27 patients suffering from liver cirrhosis, and 63 patients suffering from chronic hepatitis. The best cut-off value of AFP-IgM and free AFP for diagnosis of primary HCC were 300 AU/mL and 10 mu g/L respectively, according to the area under the curve (AUC) in this study. The sensitivity of AFP-IgM and free AFP were 64.9 and 79.7%, and the specificity were 75.6 and 80.3%, respectively, when all cases of primary HCC were analyzed, and the AUC of free AFP was larger than that of AFP-IgM (0.85 vs. 0.72, Z = 3.21). However, in case of primary HCC at early stages (stages I and II) were analyzed, the AUC of AFP-IgM was larger than that of free AFP (0.91 vs. 0.82, Z = 1.73), which demonstrated that the sensitivity of AFP-IgM and free AFP were 94.4 and 72.2%, and the specificity were 81.9 and 79.9%, respectively. When both AFP-IgM and free AFP were positive, the specificity of diagnosis of primary HCC was 89.1%, and the efficacy was 79.0%. It is concluded that either sensitivity or specificity of serum level of AFP-IgM immune complexes was higher than that of free AFP in the diagnosis of primary HCC at early stages. As there was false positive AFP-IgM existed in the patients suffering from cirrhosis and chronic hepatitis, the combination of free AFP and AFP-IgM could significantly increase specificity and decrease false negative and/or false positive in the primary HCC at early stages. J. Clin. Lab. Anal. 23:213-218, 2009. (C) 2009 Wiley-Liss, Inc.

الوصول الحر: https://lup.lub.lu.se/record/1478244Test
http://dx.doi.org/10.1002/jcla.20321Test

المؤلفون: Wu Changping, Xu Ning, Zhu yibei, Xu Bin, Jiang Jingting, Zhang Xueguan, Wu Jun, Mei Ji, Nilsson-Ehle Peter

المصدر: Journal of clinical laboratory analysis. 23(4)

مصطلحات موضوعية: Microbiology (medical), Adult, Male, medicine.medical_specialty, Pathology, Cirrhosis, Carcinoma, Hepatocellular, Luminescence, Clinical Biochemistry, Enzyme-Linked Immunosorbent Assay, Antigen-Antibody Complex, Serum alpha-fetoprotein, Gastroenterology, Young Adult, Immune system, Predictive Value of Tests, Internal medicine, medicine, Biomarkers, Tumor, Immunology and Allergy, Humans, Clinical significance, neoplasms, Tumor marker, Aged, Neoplasm Staging, Aged, 80 and over, business.industry, Biochemistry (medical), digestive, oral, and skin physiology, Liver Neoplasms, Public Health, Environmental and Occupational Health, Area under the curve, Hematology, Electrochemical Techniques, Original Articles, Middle Aged, medicine.disease, digestive system diseases, Medical Laboratory Technology, Immunoglobulin M, Hepatocellular carcinoma, Area Under Curve, embryonic structures, Female, alpha-Fetoproteins, business, Alpha-fetoprotein

الوصف: We evaluated clinical significance of serum alpha-fetoprotein (AFP)-IgM immune complexes, in comparison with free AFP, on the diagnosis of primary hepatocellular carcinoma (HCC). Serum levels of AFP-IgM immune complexes and free AFP were determined by the ELISA method and electrochemiluminescence, respectively, in 103 healthy controls, 74 patients suffering from primary HCC, 27 patients suffering from liver cirrhosis, and 63 patients suffering from chronic hepatitis. The best cut-off value of AFP-IgM and free AFP for diagnosis of primary HCC were 300 AU/mL and 10 mu g/L respectively, according to the area under the curve (AUC) in this study. The sensitivity of AFP-IgM and free AFP were 64.9 and 79.7%, and the specificity were 75.6 and 80.3%, respectively, when all cases of primary HCC were analyzed, and the AUC of free AFP was larger than that of AFP-IgM (0.85 vs. 0.72, Z = 3.21). However, in case of primary HCC at early stages (stages I and II) were analyzed, the AUC of AFP-IgM was larger than that of free AFP (0.91 vs. 0.82, Z = 1.73), which demonstrated that the sensitivity of AFP-IgM and free AFP were 94.4 and 72.2%, and the specificity were 81.9 and 79.9%, respectively. When both AFP-IgM and free AFP were positive, the specificity of diagnosis of primary HCC was 89.1%, and the efficacy was 79.0%. It is concluded that either sensitivity or specificity of serum level of AFP-IgM immune complexes was higher than that of free AFP in the diagnosis of primary HCC at early stages. As there was false positive AFP-IgM existed in the patients suffering from cirrhosis and chronic hepatitis, the combination of free AFP and AFP-IgM could significantly increase specificity and decrease false negative and/or false positive in the primary HCC at early stages. J. Clin. Lab. Anal. 23:213-218, 2009. (C) 2009 Wiley-Liss, Inc. (Less)

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c57649191449a3bcf7f07879a26a449Test
https://pubmed.ncbi.nlm.nih.gov/19623644Test

المؤلفون: Eiji Ishikawa, Kazunori Tsuda, Takeyuki Kohno, Tooru Kitamura

المصدر: Journal of Clinical Laboratory Analysis. 4:74-78

مصطلحات موضوعية: Liver Cirrhosis, Microbiology (medical), Carcinoma, Hepatocellular, Cirrhosis, Clinical Biochemistry, Immunoenzyme Techniques, Affinity chromatography, medicine, Humans, Immunology and Allergy, Detection limit, Chromatography, medicine.diagnostic_test, Chemistry, Liver Neoplasms, Biochemistry (medical), Public Health, Environmental and Occupational Health, Liter, Hematology, medicine.disease, digestive system diseases, Medical Laboratory Technology, Evaluation Studies as Topic, Hepatocellular carcinoma, Immunoassay, alpha-Fetoproteins, Alpha-fetoprotein, Conjugate

الوصف: alpha-Fetoprotein in sera from healthy subjects and patients with liver cirrhosis and hepatocellular carcinoma was fractionated into three peaks by affinity chromatography on a column of Lens culinaris agglutinin-Sepharose 4B. One peak (the first peak) was found in all the serum samples, but the other two peaks (the second and third peaks) were found only in patients with hepatocellular carcinoma. For alpha-fetoprotein in the second and third peaks, a specific enzyme immunoassay was developed. Lens culinaris agglutinin-coated polystyrene balls were incubated with alpha-fetoprotein and, after washing, with affinity-purified anti-alpha-fetoprotein Fab'-beta-D-galactosidase conjugate. beta-D-galactosidase activity bound to the polystyrene balls was assayed by fluorimetry. The maximal volume of serum that could be used without interference was 0.2 microliters, and the minimal detectable serum concentrations of alpha-fetoprotein in the second and third peaks were 3.5 mg/liter and 0.1 mg/liter, respectively. The maximal serum concentration of alpha-fetoprotein in the first peak that did not affect the detection limits of alpha-fetoprotein in the second and third peaks was 10 mg/liter. It was possible to confirm the presence of alpha-fetoprotein in the second and third peaks by a significant difference between bound beta-D-galactosidase activities in the absence and presence of alpha-methyl-D-mannoside or D-glucose. This assay may be useful for diagnosis of hepatocellular carcinoma, although further improvements remain to be made.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4baf3d488901c114f714ba42f33f3df8Test
https://doi.org/10.1002/jcla.1860040114Test

المؤلفون: Tooru Kitamura, Kazunori Tsuda, Takeyuki Kohno, Eiji Ishikawa

المصدر: Journal of clinical laboratory analysis. 4(3)

مصطلحات موضوعية: Microbiology (medical), Liver Cirrhosis, Carcinoma, Hepatocellular, Clinical Biochemistry, Chromatography, Affinity, Immunoenzyme Techniques, Agglutinin, Affinity chromatography, medicine, Biomarkers, Tumor, Immunology and Allergy, Humans, Bovine serum albumin, chemistry.chemical_classification, Detection limit, Chromatography, biology, medicine.diagnostic_test, Biochemistry (medical), Liver Neoplasms, Public Health, Environmental and Occupational Health, Hematology, beta-Galactosidase, digestive system diseases, Medical Laboratory Technology, Enzyme, chemistry, Immunoassay, biology.protein, alpha-Fetoproteins, Alpha-fetoprotein, Conjugate

الوصف: Alpha-fetoprotein in sera from patients with hepatocellular carcinoma was fractionated into three peaks by affinity chromatography on a column of Lens culinaris agglutinin-Sepharose 4B. One peak (the first peak), which passed through the column without adsorption, was found in both healthy subjects and patients with liver cirrhosis and hepatocellular carcinoma. The second and third peaks were reactive with L. culinaris agglutinin and found only in patients with hepatocellular carcinoma. For α-fetoprotein in the second and third peaks, a novel and sensitive enzyme immunoassay (immune-complex-transfer enzyme immunoassay) was developed. Alpha-fetoprotein in test serum was reacted with dinitrophenyl affinity-purified anti-α-fetoprotein IgG, and the complex formed was trapped onto affinity-purified (antidinitrophenyl bovine serum albumin) IgG-coated polystyrene balls. The polystyrene balls were washed to eliminate substance(s) other than a-fetoprotein in the test serum, and the complex was eluted from the polystyrene balls with dinitrophenyl-L-lysine. The eluted complex containing α-fetoprotein in the second and third peaks was trapped onto L. culinaris agglutinin-coated polystyrene balls and reacted with affinity-purified anti-α-fetoprotein Fab'-β-D-galactosidase conjugate. Beta-D-galactosidase activity bound to the polystyrene balls was assayed by fluorimetry. The maximal volume of serum that could be used without interference was 20μl, which was 100-fold larger than that in the previous enzyme immunoassay. As a result, the minimal detectable serum concentrations of α-fetoprotein in the second and third peaks were 0.1 mg/L and 3.5 μg/L, respectively, which were 35- and 29-fold lower than those obtained by the previous enzyme immunoassay. It was possible to confirm the presence of α-fetoprotein in the second and third peaks by a significant difference between bound β-D-galactosidase activities in the absence and presence of α-methyl-D-mannoside or D-glucose. This assay may be more useful for diagnosis of hepatocellular carcinoma than was the previous enzyme immunoassay. However, the maximal serum concentration of a-fetoprotein in the first peak that did not affect the detection limits of α-fetoprotein in the second and third peaks was 0.35 mg/L, which was also 29-fold lower than that obtained by the previous enzyme immunoassay. The detection limits of a-fetoprotein in the second and third peaks remain to be lowered without lowering the maximal serum concentration of α-fetoprotein in the first peak that does not affect the detection limit of serum α-fetoprotein in the second and third peaks.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::676ccca10344911a577800e98b573bf8Test
https://pubmed.ncbi.nlm.nih.gov/1693671Test

المؤلفون: Herbert A. Fritsche, Alan H. B. Wu, Cyrenius M. Jone, Thomas Spillman

المصدر: Journal of clinical laboratory analysis. 4(4)

مصطلحات موضوعية: Microbiology (medical), medicine.drug_class, Clinical Biochemistry, Immunoblotting, Antibody Affinity, Monoclonal antibody, Antigen, Antibody Specificity, Pregnancy, Lectins, Neoplasms, medicine, Immunology and Allergy, Humans, Antiserum, Electrophoresis, Agar Gel, biology, Biochemistry (medical), Public Health, Environmental and Occupational Health, Lectin, Antibodies, Monoclonal, Hematology, Amniotic Fluid, Molecular biology, digestive system diseases, Medical Laboratory Technology, Polyclonal antibodies, biology.protein, Affinity electrophoresis, Female, alpha-Fetoproteins, Antibody, Alpha-fetoprotein

الوصف: We adapted a method for evaluating monoclonal antibody specificity toward isoforms and examined 29 alpha-fetoprotein (AFP) antibodies from Hybritech. These antibodies were separated by affinity electrophoresis on agarose-containing erythroagglutinating phytohemagglutinin (PHA-E) followed by immunoblotting onto nitrocellulose. Under these conditions, AFP separates into four subfractions: NR (nonreactive with PHA-E), WR (reactive weakly), RS2 (strongly reactive), and RS1 (very strongly reactive). When polyclonal goat anti-AFP control was used as the test antibody, the distribution of amniotic fluid AFP was approximately 50°h, 31%, 12%, and 7% for NR, WR, RS2, and RS1, respectively. The distribution for these same bands from the serum of a patient with hepatocellular carcinoma was 39%, 31 %, 30%, and 0%; from the serum of a patient with embryonal carcinoma, it was 29%, 38%, 0%, and 33%, respectively. Twenty-six monoclonal antibodies, including the two used in the Hybritech Tandem-E assay for AFP, showed similar distribution, while the remaining three antibodies showed no reactivity to any of the AFP antigens in this system. Although we were not able to demonstrate any specificity of these anti-sera toward any single AFP isoform, use of this affinity electrophoresis system provides a model for studying isoform specificity of other AFP antibodies and other antibody-antigen systems.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e25de59de8163730e58f94d9f3284f0Test
https://pubmed.ncbi.nlm.nih.gov/1697337Test