SARS-CoV-2–specific CD8+ T cell responses in convalescent COVID-19 individuals
| العنوان: | SARS-CoV-2–specific CD8+ T cell responses in convalescent COVID-19 individuals |
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| المؤلفون: | Hermi Sumatoh, Eshan U. Patel, Maria Bettinotti, Aaron A.R. Tobian, Andrew Pekosz, Hassen Kared, Shmuel Shoham, Evan W. Newell, Thomas C. Quinn, David J. Sullivan, Daniel Carbajo, Andrew D. Redd, Kirsten Littlefield, Faris Kairi, Tania S. Bonny, Alessandra Nardin, Brian Abel, Arturo Casadevall, Evan M. Bloch, Michael G. Fehlings, Oliver Laeyendecker, Sarah E. Benner |
| المصدر: | J Clin Invest |
| بيانات النشر: | American Society for Clinical Investigation, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, T cell, Human leukocyte antigen, CD8-Positive T-Lymphocytes, Antibodies, Viral, Epitope, 03 medical and health sciences, 0302 clinical medicine, Immune system, HLA Antigens, MHC class I, medicine, Humans, Cytotoxic T cell, Neutralizing antibody, Aged, biology, SARS-CoV-2, Models, Immunological, COVID-19, Convalescence, General Medicine, Middle Aged, Antibodies, Neutralizing, 030104 developmental biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Immunology, biology.protein, Female, CD8, Research Article |
| الوصف: | Characterization of the T cell response in individuals who recover from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is critical to understanding its contribution to protective immunity. A multiplexed peptide-MHC tetramer approach was used to screen 408 SARS-CoV-2 candidate epitopes for CD8(+) T cell recognition in a cross-sectional sample of 30 coronavirus disease 2019 convalescent individuals. T cells were evaluated using a 28-marker phenotypic panel, and findings were modelled against time from diagnosis and from humoral and inflammatory responses. There were 132 SARS-CoV-2–specific CD8(+) T cell responses detected across 6 different HLAs, corresponding to 52 unique epitope reactivities. CD8(+) T cell responses were detected in almost all convalescent individuals and were directed against several structural and nonstructural target epitopes from the entire SARS-CoV-2 proteome. A unique phenotype for SARS-CoV-2–specific T cells was observed that was distinct from other common virus-specific T cells detected in the same cross-sectional sample and characterized by early differentiation kinetics. Modelling demonstrated a coordinated and dynamic immune response characterized by a decrease in inflammation, increase in neutralizing antibody titer, and differentiation of a specific CD8(+) T cell response. Overall, T cells exhibited distinct differentiation into stem cell and transitional memory states (subsets), which may be key to developing durable protection. |
| تدمد: | 1558-8238 0021-9738 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4789ad096aa74226198fe908ef04b10eTest https://doi.org/10.1172/jci145476Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4789ad096aa74226198fe908ef04b10e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15588238 00219738 |
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